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Alkalinization of phosphorylase kinase-deficient muscle during tetanic contraction

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Bioscience Reports

Abstract

The intracellular pH of resting and stimulated muscle was monitored by two independent methods: measurement of pH iniodacetate-treated homogenates of freezeclamped tissue and the absorbance at 550–443 nm of intracellular neutral red dye in vivo. During tetanic stimulation, muscle of phosphorylase kinase-deficient mice shows a transient alkalinization whereas muscle in normal mice becomes more acid under similar conditions. The alkalinization appears to be caused by abnormally rapid AMP deamination associated with adaptation to phosphorylase kinase deficiency.

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Author notes

  1. Z. H. A. Rahim

    Present address: Biochemistry Department, Faculty of Medicine, University of Malaya, Pantai Valley, 22-11, Kuala Lumpur, Malaya

Authors and Affiliations

  1. Deptartment of Biochemistry, St. George's Hospital Medical School, Cranmer Terrace, SW17 ORE, London, UK

    G. Lutaya, Z. H. A. Rahim, R. J. Shuttlewood, C. L. Bashford & J. R. Griffiths

Authors
  1. G. Lutaya
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  2. Z. H. A. Rahim
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  3. R. J. Shuttlewood
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  4. C. L. Bashford
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  5. J. R. Griffiths
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Lutaya, G., Rahim, Z.H.A., Shuttlewood, R.J. et al. Alkalinization of phosphorylase kinase-deficient muscle during tetanic contraction. Biosci Rep 1, 177–182 (1981). https://doi.org/10.1007/BF01117015

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  • DOI: https://doi.org/10.1007/BF01117015

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