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Influence of pH and ionic strength on the lysis ofMicrococcus luteus cells by hen lysozyme at low (20°C) and high (physiological, 40°C) temperature

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Abstract

From isoactivity curves (showing activity as a function of pH and ionic strength) it was found that in the pH domain 6.7–8.6 frequently used in experiments involving hen lysozyme, the pH optimum of lysis ofMicrococcus luteus ceils at low ionic strength (0.02–0.05) by the high-temperature form (40°C physiological temperature) was one to two pH units lower than that by the low-temperature form (20°C).

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Authors and Affiliations

  1. Centre de Transfusion-Réanimation de l'Armée ‘J. Julliard’, F-92141, Clamart

    J. Saint-Blancard, J. P. Maurel, J. F. Constant, J. Berthou & P. Jolles

  2. Laboratory of Proteins, University of Paris V, 45 rue des Saints-Pères, 75270, Paris Cedex 06, France

    J. Saint-Blancard, J. P. Maurel, J. F. Constant, J. Berthou & P. Jolles

Authors
  1. J. Saint-Blancard
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  2. J. P. Maurel
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  3. J. F. Constant
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  4. J. Berthou
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  5. P. Jolles
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Additional information

116th communication on lysozymes; 115th communication: Harada Y, Lifchitz A, Berthou J, & Jollès P (1981) Acta Cryst. in press.

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Saint-Blancard, J., Maurel, J.P., Constant, J.F. et al. Influence of pH and ionic strength on the lysis ofMicrococcus luteus cells by hen lysozyme at low (20°C) and high (physiological, 40°C) temperature. Biosci Rep 1, 119–123 (1981). https://doi.org/10.1007/BF01117008

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  • DOI: https://doi.org/10.1007/BF01117008

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