Abstract
Specific binding sites for somatostatin have been found in the cytosolic fractions of both parietal and non-parietal cells from rabbit gastric fundic mucosa. The stoichiometric data suggested the presence of two classes of binding sites in both types of ceils. The number of low-affinity binding sites was significantly higher in parietal cells than in non-parietal cells. The reverse was true for the high-affinity binding sites. However, the affinity of each class of binding sites was similar in the cytosolic fractions of both parietal and non-parietal ceils. It thus appears that low-affinity somatostatin binding sites are mainly located in the parietal ceils whereas the high-affinity sites occur principally in the non-parietal cells.
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References
Alumets J, Ekelund M, Elmunshid HA, Hakanson R, Loren I & Sundler F (1979) Topography of somatostatin cells in the stomach of the rat: Possible functional significance. Cell Tissue Res.202, 177–188.
Arilla E, Lopez-Ruiz P, Gonzalez-Guijarro L, Prieto JC, Gomez-Pan A & Hirst BH (1984) Characterization of somatostatin binding sites in cytosolic fraction of rat intestinal mucosa. Biochim. Biophys. Acta802, 203–208.
Bathena SJ & Recant L (1980) Somatostatin, receptors on circulating human blood cells. Horm. Metab. Res.12, 277–278.
Chayvialle JA, Descos F, Miyata M, Rayford PL & Thompson JC (1979) Molecular forms of somatostatin, in acetic extracts of pancreas and gastrointestinal mucosa. In: Hormone Receptors in Digestion and Nutrition (Rosselin G, Fromageot P & Bonfils S, eds), pp 109–114, Elsevier, Amsterdam.
Diel F, Bethge N, Schneider E & Quabbe HJ (1981) Somatostatin binding factor from chicken pancreas. J. Clin. Chem. Clin. Biochem. 19,. 99–107.
Fraker PJ & Speck JC Jr (1978) Protein and cell membrane iodinations with a sparingly soluble chloroamide 1, 3, 4, 6-tetrachloro-3α, 6α-diphenylglycoluril. Biochem. Biophys. Res. Commun.80, 849–857.
Gomez-Pan A, Reed JD, Albinus M, Shaw B, Hall R, Besser GM, Coy DH Kastin AJ & Schally AV (1975) Diiect inhibition of gastric acid and pepsin secretion by growth hormone-release inhibiting hormone in cats. Lanceti, 888–890.
Gonzalez-Guijarro L, Arilla E, Lopez-Ruiz P & Prieto JC (1984) Somatostatin binding sites in cytosolic fraction isolated from rabbit antral and fundic gastric mucosa. Regul. Peptides, in press.
Hirst BH, Arilla E, Coy DH & Shaw B (1984) Cyclic hexa- and pentapeptide somatostatin, analogues with reduced gastric inhibitory activity. Peptides5, 857–860.
Konturek SY, Tasler Y, Krol R, Dembinski A, Coy DH & Schally AV (1977) Effect of somatostatin analogs on gastric and pancreatic secretion. Proc. Soc. Exp. Biol. Med.155, 519–522.
Koop H, Coelle EF, McIntosh C, Degenhardt T, Becker HD, Arnold R & Creutzfeldt W (1980) Somatostatin and gastrin release from canine stomach. Scand. J. Gastroenterol15, 755–759.
Lehy T, Dubrasquet M, Brazeau P & Bonfils S (1982) Inhibitory effect of prolonged administration of long-acting somatostatin on gastrin-stimulated fundic epithelial cell growth in the rat. Digestion24, 246–255.
Leitner JW, Rifkin RM, Maman A & Sussan KE (1979) Somatostatin binding to pituitary plasma membranes. Biochem. Biophys. Res. Commun.87, 919–927.
Lewin MJM, Cheret AM, Soumarmon A & Girodet J (1984) Méthode pour l'isolement et le tri des cellules de la muqueuse fundique de rat. Biol. Gastroenterol.7, 139–144.
Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275.
Reyl-Desmars F & Lewin MJM (1982a) Intracellular receptor for somatostatin in gastric mucosal cells: Decomposition and reconstitution of somatostatin-stimulated phosphoprotein phosphatases. Proc. Natl. Acad. Sci. U.S.A.79, 978–982.
Reyl-Desmars F & Lewin MJM (1982b) Evidence for an intracellular somatostatin receptor in pancreas: A comparative study with reference to gastric mucosa. Biochem. Biophys. Res. Commun.109, 1324–1331.
Scatchard G (1949) The attraction of protein for small molecules and ions. Ann. N. Y. Acad. Sci.51, 660–762.
Schrumpf E, Vatn MH, Hanssen KF & Myren JM (1978) A small dose of somatostatin, inhibits the pentagastrin stimulated gastric secretion of acid, pepsin and intrinsic factor in man. Clin. Endocrinol.8, 391–395.
Tange A (1983) Distribution of peptide-containing endocrine cells and neurons in the gastrointestinal tract of the dog: Immunohistochemical studies using antisera to somatostatin, substance P, vasoactive intestinal polypeptide, Met-enkephalin and neurotensin. Biomed. Res.4, 9–24.
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Arilla, E., Pilar López-Ruiz, M., Gonzalez-Guijarro, L. et al. Somatostatin binding sites in cytosolic fractions of parietal and non-parietal cells from rabbit fundic mucosa. Biosci Rep 5, 321–328 (1985). https://doi.org/10.1007/BF01116904
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DOI: https://doi.org/10.1007/BF01116904