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Antibodies to guanylic acid: Fractionation and specificities

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Bioscience Reports

Abstract

Guanylic-acid-specific antibodies were elicited in rabbits) using as immunogen pG linked through 5′-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides) nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments . Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of 107 M−1 at 4°C Binding of the antibodies to3H-RNA is G-specifiC. The antibodies do not bind to32P-ssDNA or32P-dsDNA. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions) though specific to pG) differ in their cross-reactivities to nucleosides and nucleotides.

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Abbreviations

G:

guanosine

pG:

guanosine 5′-phosphate (similarly for other nucleotides)

dpG:

deoxyriboguanosine 5′-phosphate

ssDNA:

single-stranded DNA

dsDNA:

double-stranded DNA

Tg:

thyroglobulin

BSA:

bovine serum albumin

RSA:

rabbit serum albumin

EDC:

1-ethyl-1-3 (3-dimethylisopropyl) carbodiimide

Ac-Lys-NHMe:

N-α-acetyl L-lysine N-methylamide

Ac-Lys-(pG)-NHMe:

pG coupled through the phosphate to the ε-amino group of Ac-Lys-NHMe

AH-Sepharose-pG:

aminohexyl-Sepharose-pG

TBS:

Tris-buffered saline

SLE:

systemic lupus erythematosus

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Author notes

  1. S. Iswari

    Present address: Department of Physiological Chemistry, University of Wisconsin, 53706, Madison, Wisconsin, USA

Authors and Affiliations

  1. Department of Biochemistry and ICMR Center for Cell Biology and Genetics, Indian Institute of Science, 560012, Bangalore, India

    S. Iswari & T. M. Jacob

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  1. S. Iswari
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  2. T. M. Jacob
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Iswari, S., Jacob, T.M. Antibodies to guanylic acid: Fractionation and specificities. Biosci Rep 4, 1027–1036 (1984). https://doi.org/10.1007/BF01116695

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