Abstract
Arrhenius plots of fluoride- and guanine-nucleotide-stimulated adenylate cyclase activity were linear in adipocyte plasma membranes from lean and obese (ob/ob) mice . Arrhenius plots of isoprenaline-stimulated adenylate cyclase activity in hepatic plasma membranes biphasic in both groups. The results were biphasic in membranes from Jean mice but linear in membranes from obese mice. In contrast, Arrhenius plots of glucagon-stimulated adenylate cyclase activity in hepatic plasma membranes were biphasic in both groups. The results suggest that the coupling between the β-receptor and the regulatory unit of adenylate cyclase, which has been observed to be defective in adipocyte plasma membranes from obese mice, is influenced by a different lipid environment in membranes from obese animals.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Begin-Heick N (1980) Can. J. Biochem.58, 1033–1038.
Bogartz RS (1968) Psychol. Bull.70, 749–755.
Bradford MM (1976) Anal. Biochem.72, 248–254.
Brown BL, Ekins RP & Albano JDM (1972) Adv. Cyclic Nucleotide Res.2, 25–40.
Colley CM & Metcalfe JC (1972) FEBS Lett.24, 241–246.
Cuendot G, Loten E, Jeanrenaud B & Renold A (1976) J. Clin. Invest58, 1078–1088.
Dehaye JP, Winand J & Christophe J (1978) Diabetologia13, 533–561.
Dipple I & Houslay MD (1978) Biochem. J.174, 179–190.
Enser M (1970) Nature226, 175–177.
French RR, York DA, Portman JM & Isaacs K (1983) Comp. Biochem. Physiol.76B, 309–319.
French RR & York DA (1984) Diabetologia26, 466–472.
Gordon LM, Sauerheber RD, Esgate JA, Dipple I, Marchmont RJ & Houslay MD (1980) J. Biol. Chem.255, 4519–4527.
Hesketh TR, Smith GA, Houslay MD, McGill KA, Birdsall NJM, Metcalfe JC & Warren GB (1976) Biochemistry15, 4145–4151.
Himms-Hagen J & Desautels M (1978) Biochem. Biophys. Res. Commun.83, 628–634.
Houslay MD, Hesketh TR, Smith GA, Warren GB & Metcalfe JC (1976) Biochim. Biophys. Acta436, 495–504.
Houslay MD & Gordon LM (1983) In: Current Topics in Membranes and Transport (Kleinzeller A & Martin BR, eds), pp 179–231, Academic Press, New York, London.
Hyslop PA & York DA (1980) Biochem. Biophys. Res. Commun.92, 819–824.
Kirilovsky J & Schramm M (1983) J. Biol. Chem.258, 6841–6849.
Laudat MH & Pairault J (1975) Eur. J. Biochem.56, 583–589.
Lee AG, Birdsall NJM, Metcalfe JC, Toon PA & Warren GB (1974) Biochemistry132, 3699–3705.
Lowry OH, Rosebrough NJ, Farr Al & Randall RJ (1951) J. Biol. Chem.193, 265–275.
Needham L & Houslay MD (1982) Biochem. J.206, 89–95.
Needham L, Whetton AD & Houslay MD (1982) FEBS Lett.140, 85–88.
Pfeuffer T (1979) FEBS Lett.101, 85–89.
Ray TK (1970) Biochim. Biophys. Acta647, 302–306.
Rodbell M (1967) J. Biol. Chem.242, 5744–5750.
Rodbell M (1980) Nature (London)284, 17–22.
Sauerheber R, Esgate JA & Kuhn CE (1982) Biochim. Biophys. Acta.691, 115–124.
Wunderlich F, Ronai A, Speth V, Seelig J & Blume A (1975) Biochemistry14, 3730–3735.
York DA, Bray GA & Yukimara Y (1978) Proc. Natl. Acad. Sci. U.S.A.75, 477–487.
York DA, Hyslop PA & French R (1982) Biochem. Biophys. Res. Comm.106, 1478–1483.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
French, R.R., York, D.A. Arrhenius plot characteristics of adipocyte-plasma-membrane adenylate cyclase activity in lean and genetically obese (ob/ob) mice. Biosci Rep 4, 1001–1008 (1984). https://doi.org/10.1007/BF01116692
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01116692