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Studies on the involvement of calcium and calmodulin in the action of growth-hormone-releasing factor

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Bioscience Reports

Abstract

A possible role for Ca 2+ and calmodulin in the action of growth-hormone-releasing factor (GHRF) was investigated . Low extracellular Ca2+ (<100 μM), methoxyverapamil, flunarizine, cinnarizine, and Co2+ decreased both basal and GHRF-stimulated growth-hormone secretion, but did not totally inhibit GHRF-stimulation secretion. A calmodulin antagonist, W7, abolished GHRF-stimulated GH secretion, with no effect on basal secretion. It is suggested that GHRF may act primarily by elevating cellular cyclic AMP, which may then modulate calcium mobilization or flux; the increased intracellular Ca2+ concentrations may then activate calmodulin.

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Author notes

  1. Janet E. Merritt

    Present address: Department of Pharmacology and Toxicology, Medical College of Virginia, Box 613 MCV Station, 23298-0001, Richmond, Virginia, USA

Authors and Affiliations

  1. Department of Human Metabolism & Clinical Biochemistry, University of Sheffield Medical School, Beech Hill Road, S10 2RX, Sheffield, UK

    Janet E. Merritt, Pauline R. M. Dobson, Richard J. H. Wojcikiewicz, John G. Baird & Barry L. Brown

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  1. Janet E. Merritt
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  2. Pauline R. M. Dobson
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  3. Richard J. H. Wojcikiewicz
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  4. John G. Baird
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  5. Barry L. Brown
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Merritt, J.E., Dobson, P.R.M., Wojcikiewicz, R.J.H. et al. Studies on the involvement of calcium and calmodulin in the action of growth-hormone-releasing factor. Biosci Rep 4, 995–1000 (1984). https://doi.org/10.1007/BF01116691

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  • DOI: https://doi.org/10.1007/BF01116691

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