Abstract
Rat liver dolichol and dolichyl-P were labeled by injection of [3H]mevalonate into the portal vein and their rates of synthesis and breakdown determined. In the initial phase the radioactivity appeared in α-unsaturated polyprenols. Subsequent saturation required 90 min. The half-lives of dolichols in microsomes were between 80 and 118 h, and shorter dolichols had shorter values of T1/2. The half-lives of dolichols in lysosomes were between 115 and 137 h, while microsomal dolichyl-P exhibited a T1/2 of 32 h. Injected dolichol was recovered in the lysomes of hepatocytes and exhibited a rate of breakdown which was slower than that of the endogenous compound. These results indicate differences in the catabolism of dolichol at different subcellular locations, as well as differences between the catabolism of dolichol and dolichyl-P.
References
Hemming, F. W. (1983). In:Biosynthesis of Isoprenoid Compounds (Porter, J. W. and Spurgeon, S. L., eds.), Vol. 2, Wiley & Sons, New York, pp. 305–354.
Rupar, C. A. and Carroll, K. K. (1978).Lipids 13:291–293.
Tollbom, Ö. and Dallner, G. (1986).Br. J. Exp. Path. 67:757–764.
Struck, D. K. and Lennarz, W. J. (1980). In:The Biochemistry of Glycoproteins and Proteoglycans (Lennarz, W. J., ed.), Plenum Press, New York, pp. 35–73.
Valtersson, C., van Duyn, G., Verkleij, A. J., Chojnacki, T., de Kruijff, B. and Dallner, G. (1985).J. Biol. Chem. 260:2742–2751.
de Ropp, J. S. and Troy, F. A. (1985).J. Biol. Chem. 260:15669–15674.
Marino, M., Bruscalupi, G., Spagnuolo, S., Leoni, S., Mangiantini, A., Trentalance, A. and Hemming, F. W. (1986).Biosci. Rep. 6:409–412.
Edlund, C., Chojnacki, T. and Dallner, G. (1983).Biochem. Biophys. Res. Commun. 115:917–923.
Edlund, C., Ganning, A. E. and Dallner, G. (1986).Chem.-Biol. Interactions 57:255–270.
Eggens, I., Chojnacki, T., Kenne, L. and Dallner, G. (1983).Biochim. Biophys. Acta 751:355–368.
Pullarkat, R. K. and Reha, H. (1982).J. Biol. Chem. 257:5991–5993.
Wolfe, L. S., Ng Ying Kin, N. M. K., Palo, J. and Haltia, M. (1982).Lancet ii:99.
Rossignol, D. P., Scher, M., Waechter, C. J. and Lennarz, W. J. (1983).J. Biol. Chem. 258:9122–9127.
Eggens, I., Eriksson, L. E., Chojnacki, T. and Dallner, G. (1984).Cancer Res. 44:799–805.
Keller, R. K., Armstrong, D., Crum, F. C. and Koppang, N. (1984).J. Neurochem. 42:1040–1047.
Adair, W. L., Cafmeyer, N. and Keller, R. K. (1984).J. Biol. Chem. 259:4441–4446.
Ekström, T. J., Chojnacki, T. and Dallner, G. (1984).J. Biol. Chem. 259: 10460–10468.
Keenan, R. W., Fischer, J. B. and Kruczek, M. E. (1977).Arch. Biochem. Biophys. 179:634–642.
Rip, J. W. and Carroll, K. K. (1985).Biochem. J. 227:705–710.
Adair, W. L. and Keller, R. K. (1982).J. Biol. Chem. 257:8990–8996.
Mankowski, T., Jankowski, W., Chojnacki, T. and Franke, P. (1976).Biochemistry 15:2125–2130.
Keenan, R. W. and Kruczek, M. (1975).Anal. Biochem. 69:504–509.
Elmberger, P. G., Kalén, E., Appelkvist, E. L. and Dallner, G. (1987).Eur. J. Biochem. 168:1–11.
Keller, R. K., Jehle, E. and Adair, W. L. (1982).J. Biol. Chem. 257:8985–8989.
Chojnacki, T. and Dallner, G. (1983).J. Biol. Chem. 258:916–922.
Ekström, T. J., Chojnacki, T. and Dallner, G. (1987).J. Biol. Chem. 262: 4090–4097.
Eriksson, L. C. and Dallner, G. (1973).FEBS Lett. 29:351–354.
Connelly, L. W. and Keller, R. K. (1984).Biosci. Rep. 4:771–776.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Edlund, C., Brunk, U., Chojnacki, T. et al. The half-lives of dolichol and dolichyl phosphate in rat liver. Biosci Rep 8, 139–146 (1988). https://doi.org/10.1007/BF01116458
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01116458