Abstract
We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.
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Kitamoto, T., Hikita, K., Tashima, T. et al. Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis. Biosci Rep 6, 459–465 (1986). https://doi.org/10.1007/BF01116137
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DOI: https://doi.org/10.1007/BF01116137