Abstract
The effects of experimental uremia on the concentration of vasoactive intestinal peptide (VIP) in duodenum as well as on the interaction of this neuropeptide with the corresponding epithelial cells were studied in rats. Duodenal VIP concentration was significantly decreased in uremic rats as compared to control animals. The specific binding of VIP to duodenal epithelial cells increased in rats with uremia due to an increase in the number of VIP receptors rather than a change in the binding affinity or in the extent of VIP degradation. On the other hand, the efficacy but not the potency of VIP upon cyclic AMP generation varied in parallel to that observed at the receptor level.
References
Castrup, H. J., Lohrs, U. and Eder, M. (1970)Virchows Arch. 349:357–367.
McDermott, F. T., Nayman, J. and De Boer, W. G. (1971)Ann. Surg. 174:274–282.
McDermott, F. T., Galbraith, A. J. and Dalton, M. K. (1974)Gastroenterology 66: 235–239.
McDermott, F. T., Dalton, M. K. and Galbraith, A. J. (1974)Cell Tiss. Kinet. 7: 31–36.
McDermott, F. T., Dalton, M. K. and Galbraith, A. J. (1974)Digest. Dis. 19:1116–1119.
Boynton, A. and Whitfield, J. F. (1983)Adv. Cyclic Nucleot. Res. 15:193–294.
Besson, J., Laburthe, M., Bataille, D., Dupont, C. and Rosselin, G. (1978)Acta Endocrinol. 87:799–810.
Laburthe, M., Prieto, J. C., Amiranoff, B., Dupont, C., Hui Bon Hoa, D. and Rosselin, G. (1979)Eur. J. Biochem. 96:239–248.
Prieto, J. C., Laburthe, M. and Rosselin, G. (1979)Eur. J. Biochem. 96:229–237.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol. Chem. 193:265–275.
Freychet, P., Roth, J. and Neville, D. M. (1971)Proc. Natl. Acad. Sci. USA 68: 1833–1837.
Gilman, A. G. (1970)Proc. Natl. Acad. Sci. USA 67:305–312.
Laburthe, M., Bataille, D. and Rosselin, G. (1977)Acta Endocrinol. 84:588–599.
Scatchard, G. (1949)Ann. N. Y. Acad. Sci. 51:660–672.
Bozzini, C. E., Devoto, F. C. H. and Tomio, J. M. (1966)J. Lab. Clin. Med. 68:411–419
McDermott, F. T., Nayman, J. and De Boer, W. G. (1968)Ann. Surg. 168:142–146.
Bastie, M. J., Balas, D., Laval, J., Bertrand, J. and Ribet, A. (1985)Acta Anat. 124:133–144.
Amiranoff, B. and Rosselin, G. (1982) In:Vasoactive Intestinal Peptide (Said, S. I., Ed.) Raven Press, New York, pp. 307–322.
Helinek, T. G., Sadel, S. and Card, J. F. (1984)Metabolism 33:158–163.
Piga, M., Altieri, P., Floris, A., Barraca, A., Tagleri, G., Madeddu, G., Bolasco, F., Gervasi, F., Piras, M. R., Serra, A. R. and Balestrieri, A. (1984)J. Nucl. Med. All. Sci. 28:77–80.
Henriksen, J. H., Staun-Olsen, P., Borg-Mogensen, N. and Fahrenkrug, J. (1986)Eur. J. Clin. Invest. 16:211–216.
Cohen, G. A., Goffinet, J. A., Donabedian, R. K. and Conn, H. O. (1976)Ann. Int. Med. 84:275–280.
Tvedegaard, E., Poulsen, E., Vilstrup, H. and Thomsen, H. K. (1984)Experientia 40:1255–1256.
Rosselin, G., Laburthe, M., Bataille, D., Prieto, J. C., Dupont, C., Amiranoff, B., Broyart, J. P. and besson, J. (1980) In:Hormones and Cell Regulation. Vol. 4 (Dumont, J. and Nuñez, J., Eds.) Elsevier/North Holland, Amsterdam, pp. 311–330.
Fernández-Moreno, M. D., Díaz-Juárez, J. L., Arilla, E. and Prieto, J. C. (1985)Horm. Metab. Res. 17:289–292.
Jaffe, R. H. and Laing, D. R. (1934)Arch. Int. Med. 53:851–864.
Kirkegaard, P., Lundberg, J. M., Poulsen, S. S., Olsen, P. S., Fahrenkrug, J., Hokfelt, T. and Christiansen, J. (1981)Gastroenterology 81:872–878.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fernández-Moreno, M.D., Arilla, E. & Prieto, J.C. The effects of experimental uremia in rats on duodenal VIP levels and the interaction of VIP with duodenal epithelial cells. Biosci Rep 9, 207–212 (1989). https://doi.org/10.1007/BF01115997
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01115997