Abstract
Ruthenium red increased specific insulin binding to isolated adipocytes 5.4 fold and 2.6 fold over binding determined in the absence and presence of Ca2+ and Mg2+. The increase in insulin binding was not accompanied by an increase in insulin sensitivity. The lack of effect of ruthenium red on insulin action argued strongly against an increase in intracellular Ca2+ as a potential messenger/transducer of insulin action and suggested that the enhancing effect of Ca2+ on insulin action was a result of increased receptor affinity.
Abbreviations
- RR:
-
ruthenium red
- BSA:
-
bovine serum albumin
- Hepes:
-
4-(2-hydroxyethyl-1-piperazineethane-sulphonic acid
References
Cuatracases, R. (1971).J. Biol. Chem. 246:7265–7274.
Desai, K. S., Zinman, G. S., Steiner, G., and Hollenburg, C. H. (1978).Can. J. Biochem. 56:843–848.
Williams, P. F., and Turtle, J. R. (1981).Biochim. Biophys. Acta 676:113–117.
Eckel, J., and Reinauer, H. (1984).Diabetes 33:214–218.
Akhtar, R. A., and Perry, M. C. (1979a)Biochim. Biophys. Acta 585:107–117.
Akhtar, R. A., and Perry, M. C. (1979b).Biochim. Biophys. Acta 585:117–127.
Siddle, K., and Hales, C. M. (1980).Horm. Metab. Res. 12:509–515.
Williams, P. F., Caterson, I. D., and Turtle, J. R. (1984).Biochim. Biophys. Acta 797: 27–33.
Williams, P. F., and Turtle, J. R. (1984).Diabetes 33:1106–1111.
Kissebah, A. H., Clarke, P., Vydelingum, N., Hope-Gill, H., Tulloch, B., and Fraser, T. R. (1975).Eur. J. Clin. Invest. 5:339–349.
Clausen, T. (1980).Cell Calcium 1:311–325.
Schaltzman, H. J., and Tschabold, M. (1971).Experienta. 27:59–61.
Watson, E. L., Vincenzi, F. F., and Davies, P. W. (1971).Biochim. Biophys. Acta 249: 606–610.
Hinds, T. R., Raess, B. U., and Vincenzi, F. F. (1981).J. Membrane Biol. 58:57–65.
Martin, R. B., and Richardson, F. S. (1979).Q. Rev. Biophys. 12: 181–209.
Mikkelson, R. B. (1976).Biol. Membr. 3:153–190.
Luft, J. H. (1971).Anat. Rec. 171:347–368.
Luft, J. H. (1971).Anat. Rec. 171:369–416.
McCormack, J. G., and England, P. J. (1983).Biochem. J. 214, 581–585.
Peng, C., Kane, J. J., Straub, K. D., and Murphy, M. L. (1980).J. Cardiovascular Pharmacol. 2:45–54.
Stucki, J. W., and Ineichen, E. A. (1974).Eur. J. Biochem. 12:328–335.
Plehwe, W. E., Williams, P. F., Caterson, I. D., Harrison, L., and Turtle, J. R. (1983).Biochem. J. 214:361–366.
Williams, P. F., and Turtle, J. R. (1979).Biochim. Biophys. Acta 579:367–374.
Vassington, F. D., Gazzotti, P., Tiozzo, R., and Carafoli, E. (1972).Biochim. Biophys. Acta 256:43–54.
Raess, B. U., and Vincenzi, F. F. (1980).Molecular Pharmacology 18:253–258.
Bygrave, F. L. (1978).Biol. Rev. 53:43–79.
Broekemeier, K. M., Schmid, P. C., Schmid, H. H. O., and Pfeiffer, D. R. (1985).J. Biol. Chem. 260:105–113.
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Williams, P.F., Plehwe, W.E. & Turtle, J.R. Dissociation of insulin binding from insulin stimulation of 2-deoxyglucose transport by ruthenium red. Biosci Rep 6, 317–322 (1986). https://doi.org/10.1007/BF01115161
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DOI: https://doi.org/10.1007/BF01115161