Abstract
Total half-cystine residues in proteins of pig liver chromatin have been measured. About half of them are present in the reduced state. Thiol groups of non-historic chromatin proteins, which amount to about 40 nmol/mg of protein, are preferentially located in chromatin fragments which are more easily solubilised either by DNAse I or by DNAse II. The data obtained are compatible with an involvement of SH and SS groups in chromatin structure and function.
References
Sumner, A. T. (1983). InKew Chromosome Conference II (P. E. Brandham and M. D. Bennet, Eds), George Allen and Unwin, London, pp. 1–9.
Gronow, M., and Thackrah, T. M. (1973).Arch. Biochem. Biophys. 158:377–386.
Gronow, M. and Thackrah, T. M. (1974).Chem. Biol. Interactions 9:225–236.
Blobel, G. and Potter, V. R. (1966).Science 154:1162–1165.
Rickwood, D., Riches, P. G., and MacGillivray, A. J. (1973).Biochim. Biophys. Acta 299: 162–171.
Bloom, K. S., and Anderson, J. M. (1978).J. Biol. Chem. 253:4446–4450.
MacGillivray, A. J. (1976). InSubnuclear Components (G. D. Birnie, Ed.), Butterworths, London, 209–265.
Cuatrecasas, P. (1970).J. Biol. Chem. 245:3059–3065.
Duhl, D. M., Briggs, R. C., and Hnilica, L. S. (1984).Arch. Biochem. Biophys. 231: 303–308.
Gottesfeld, J. M., Garrard, W. T., Bagi, G., Wilson, R. F., and Bonner, J. (1974)Proc. Nat. Acad. Sci. USA 71:2193–2197.
Spencer, R. L., and Wold, F. (1969).Anal. Biochem. 32:185–190.
Grassetti, D. R., and Murray, J. F., Jr. (1967).Arch. Biochem. Biophys. 119:41–49.
Laemmli, U. K. (1970).Nature 227:680–685.
Lowry, O. H., Rosebrough, M. J., Farr, A. L., and Randall, R. C. (1951).J. Biol. Chem. 193:265–275.
Ruiz-Carrillo, A., and Allfrey, V. G. (1973).Arch. Biochem. Biophys. 154:185–191.
Goodwin, G. H., Nicolas, R. H., and Johns, E. W. (1975).Biochim. Biophys. Acta 405: 280–291.
Tasheva, B., and Dessev, G. (1983).Anal. Biochem. 129:89–102.
Ochs, D. (1983).Anal. Biochem. 135:470–474.
Walker, J. M., Goodwin, G. H., and Johns, E. W. (1976).Eur. J. Biochem. 62:461–469.
Tsanev, R. (1983).Mol. Biol. Rep. 9:9–17.
Norman, G. L., and Bekhor, I. (1981).Biochem. 20:3568–3578.
Leibovitch, S. A., Leibovitch, M. P., Hillion, J., Kruh, J. and Harel, J. (1983).Nucl. Acid Res. 11:4035–4047.
Bryan, S. E., Simons, S. J., Vizard, D. L., and Hardy, K. J. (1976).Biochem. 15:1667–1676.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ferraro, A., Giartosio, A., Eufemi, M. et al. Thiol proteins in chromatin. Biosci Rep 6, 257–263 (1986). https://doi.org/10.1007/BF01115154
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01115154