Conclusions
The use of diethylpyrocarbonate to inhibit endogenous ribonuclease in sheep pancreas allows the detection of protein-disulphide-isomerase activity in homogenates, at specific activities of up to 4 units/g. This is higher than the specific activity in sheep liver homogenates (about 2 units/g) or in homogenates of other sheep tissues (16). It is thus evident that high levels of protein-disulphide-isomerase activity are present in sheep pancreas. This is consistent both with the postulated general role of protein disulphide-isomerase in protein biosynthesis (10,11) and with the in vitro action of the enzyme on its conventional substrate scrambled ribonuclease, since pancreas is the major site of ribonuclease synthesis.
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Hillson, D.A., Anderson, J. Detection of protein disulphide-isomerase in sheep pancreas fractions. Biosci Rep 2, 343–349 (1982). https://doi.org/10.1007/BF01115120
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DOI: https://doi.org/10.1007/BF01115120