Abstract
Proteins fromSaccharomyces cerevisiae were tested for their crossreactivity with antibodies raised against nuclear lamina proteins of Ehrlich Ascites Tumour cells. The results of the immunoblotting experiments and ELISA suggest the existence of at least two proteins (65 and 59 kDa) which are immunologically related to the nuclear lamina proteins of higher eukaryotes.
References
Krohne, G. and Benavente, R. (1986).Exptl. Cell Res. 162:1–10.
Gerace, L., Blum, A. and Blobel, G. (1978).J. Cell Biol. 79:546–566.
Gerace, L. and Blobel, G. (1980).Cell 19:277–287.
Aebi, U., Cohn, J., Buhle, L. and Gerace, L. (1986).Nature (USA)323: 560–564.
Ballario, P. and Pedone, F. (1984). Abstr. of XIIth Intern. Conf. of Yeast Gen. and Mol. Biol., Edinburgh, UK, pp. 275.
Sherman, F., Fink, G. R. and Luking, H. (1970). In:Methods in Yeast Genetics, Cold Spring Harbor, New York, pp. 53.
Laemmli, U. K. (1970).Nature (London) 227:680–685.
Krachmarov, Ch., Tasheva, B., Markov, D., Hancock, R. and Dessev, G. (1986).J. Cell. Biochem. 30:351–359.
Galcheva-Gargova, Z., Marinova, E. and Koleva, S. (1987).Plant, Cell and Env. (in press).
Gerace, L. and Blobel, G. (1982).Cold Spring Harbor Symp. Quant. Biol. 46:967–978.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Galcheva-Gargova, Z., Stateva, L. Immunological identification of two lamina-like proteins inSaccharomyces cerevisiae . Biosci Rep 8, 287–291 (1988). https://doi.org/10.1007/BF01115046
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01115046