Abstract
Total RNA from post mortem human caudate nucleus, cerebellum, cerebral cortex and pheochromocytoma tissues has been prepared. Northern blot analysis, using a single-stranded human proenkephalin A antisense probe (cRNA), revealed the existence of two different proenkephalin A-like sequences in the human caudate nucleus and pheochromocytoma RNA extracts of approximately 1400 and 1000 nucleotides in length respectively, whereas no specific RNA bands could be detected in the cortex and only the 1400 nucleotide band was present in the cerebellum. Under highly stringent hybridization conditions, the proenkephalin A-like RNA bands still appear, indicating that the detected RNA species have either identical or a closely related sequence to that of the wellcharacterized human proenkephalin A mRNA sequence.
This is a preview of subscription content, log in via an institution to check access.
References
Comb, M., Seeburg, P. H., Adelman, J., Eiden, L. and Herbert, E. (1982).Nature 295:663–666.
Noda, M., Furutani, Y., Takahasi, H., Toyosato, M., Hirose, T., Inayama, S., Nakanishi, S. and Numa, S.(1982).Nature 295:202–206.
Yanase, T., Nawata, H., Haji, M., Kato, K. and Ibayashi, H. (1987).Mol. Cell. Endocrinol. 51:237–241.
Pittius, C. W., Kley, N., Loeffer and Höllt, V. (1985).EMBO J. 4:1257–1260.
Jingami, H., Nakanishi, S., Imura, H. and Numa, S. (1984).Eur. J. Biochem. 142:441–447.
Cunningham, M. W. and Mundy, C. R. (1987).Nature 326:723–724.
Monstein, H.-J., Folkesson, R. and Terenius, L. (1987).Life Sci. 39:2237–2241.
Chomczynski, P. and Sacchi, N. (1987).Anal. Biochem. 162:156–159.
Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982).Molecular Cloning. A Laboratory Manual (Cold Spring Harbor Laboratory).
Johnson, D. A., Gautsch, J. W., Sportsman, J. R. and Elder, J. H. (1984).Gene Anal. Tech. 1:3–8.
Melton, D. A., Krieg, P. A. Rebagliati, M. R., Maniatis, T., Zinn, K. and Green, M. R. (1984).Nucleic Acids Res. 12:7035–7069.
Kilpatrick, D. L., Howells, R. D., Noe, M., Bailey, L. C. and Udenfriend, S. (1985).Proc. Natl. Acad. Sci. USA 82:7467–7469.
Kilpatrick, D. L., Borland, K. and Jin, D. F. (1987).Proc. Natl. Acad. Sci. USA 84:5695–5699.
Yoshikawa, K., Williams, C. and Sabol, S. L. (1984).J. Biol. Chem. 259:14301–14308.
Civelli, O., Birnberg, N. and Herbert, E. (1982).J. Biol. Chem. 257:6783–6787.
Comb, M., Rosen, H., Seeburg, P., Adelman, J. and Herbert, E. (1983).DNA 2:213–229.
Mason, P. J., Jones, M. B., Elkington, J. A. and Williams, J. G. (1985).EMBO J. 4:205–211.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Monstein, HJ., Geijer, T. A highly sensitive northern blot assay detects multiple proenkephalin a-like mRNAs in human caudate nucleus and pheochromocytoma. Biosci Rep 8, 255–261 (1988). https://doi.org/10.1007/BF01115042
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01115042