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Immunoperoxidase localization of type X collagen in chick tibiae

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Bioscience Reports

Abstract

Type X collagen was prepared from medium of long-term cultures of embryonic chick tibiotarsal chondrocytes. Antibodies to type X collagen were raised and used in immunoperoxidase localization studies with embryonic and growing chick tibiotarsus. Strong anti-type X collagen reactivity was detected mainly in the region of hypertrophic chondrocytes, and to a lesser extent in the zone of calcified cartilage. No reactivity was detected in the proliferative zone nor the superficial layer of the cartilage growth plate. These results suggest that type X collagen may play a key role in matrix calcification during growth and development of the skeletal system.

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Authors and Affiliations

  1. Department of Biochemistry, Medical School, University of Manchester, M13 9PT, Manchester, UK

    Alvin P. L. Kwan & Michael E. Grant

  2. Department of Pathology, Medical School, University of Manchester, M13 9PT, Manchester, UK

    Anthony J. Freemont

Authors
  1. Alvin P. L. Kwan
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  2. Anthony J. Freemont
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  3. Michael E. Grant
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Kwan, A.P.L., Freemont, A.J. & Grant, M.E. Immunoperoxidase localization of type X collagen in chick tibiae. Biosci Rep 6, 155–162 (1986). https://doi.org/10.1007/BF01115001

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  • DOI: https://doi.org/10.1007/BF01115001

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