Abstract
Electron paramagnetic resonance (EPR) measurements using various fatty acid spin labels were performed on membranes of intact human erythrocytes at physiological, and at low ionic strength. In the case of spin probes bearing the nitroxide near the polar head group, a less restricted motion at low ionic strength was seen than with those labels with a nitroxide deeper within the hydrophobic tail of the membrane. Although these data clearly show an influence of ionic strength on membrane structure, and possibly a modified protein-lipid interaction, they cannot be simply discussed in terms of an altered membrane fluidity.
References
Cevs, G., Watts, A. and Marsh, D. (1981).Biochemistry 20:4955–4965.
Corda, D., Pasternak, C. and Shinitzky, M. (1982).J. Membr. Biol. 65:235–242.
Weinstein, J. N., Blumenthal, R., van Renswoude, J., Kempf, C. and Klausner, R. D. (1982).J. Membr. Biol. 66:203–212.
Edidin, M., Wei, T. and Holmberg, S. (1980).Ann. N.Y. Acad. Sci. 339:1–7.
Koyonova, R. D. and Tenchov, B. G. (1983).Biochim. Biophys. Acta 727:351–356.
Wojtczak, L. and Nelecz, M. J. (1979).Eur. J. Biochem. 94:99–107.
Pethica, B. A. (1984). In:Cell Fusion, Ciba Foundation symposium 103, pp. 1–8, Pitman Books, London.
Schäfer, G. and Rowohl-Quinshoudt, G. (1975).FEBS Lett. 59:48–51.
Glaser, R. (1979).J. Membr. Biol. 51:217–228.
Glaser, R. (1982).J. Membr. Biol.,66:79–85.
Glaser, R. and Donath, J. (1984).Bioelectrochem. Bioenergetics 13:71–83.
Herrmann, A., Müller, P. and Glaser, R. (1985).Biosci. Rep. 5:417–423.
Doulah, F. A., Coakley, W. T. and Tilley, D. (1984).J. Biol. Phys. 12:44–51.
Lange, Y., Matthies, H. and Steck, T. L. (1984).Biochim. Biophys. Acta 769:551–562.
Donath, E. and Arndt, R. (1984).Gen. Physiol. Biophys. 3:239–249.
Herrmann, A., Lentzsch, P., Lassmann, G., Ladhoff, A. M. and Donath, E. (1985).Biochim. Biophys. Acta 812:277–285.
Herrmann, A., Arnold, K., Lassmann, G. and Glaser, R. (1982).Acta biol. med. germ. 41:289–298.
Griffith, O. H. and Jost, P. (1976). In:Spin Labeling, Theory and Applications (Berliner, L. J., ed.), pp. 454–523, Academic Press, New York.
Butler, K. W. and Smith, I. C. P. (1978).Can. J. Biochem. 56:117–122.
Bernhardt, I., Donath, E. and Glaser, R. (1984).J. Membr. Biol. 78:249–255.
Hoffman, J. F. and Laris, P. C. (1974).J. Physiol. 239:519–552.
Nwafor, A. and Coakley, W. T. (1985).Biochem. Pharm. 34:3329–3336.
Schreier, S., Polnaszek, C. F. and Smith, I. C. P. (1978).Biochem. Biophys. Acta 515: 395–436.
Yamaguchi, T., Takehara, H., Shibata, E. (1983).Biochim. Biophys. Acta 736:150–156. 156.
Devaux, P. F. and Seigneuret, M. (1985).Biochim. Biophys. Acta 822:63–125.
O'Shea, P. S., Feuerstein-Thelen, S. and Azzi, A. (1984).Biochem. J. 220:795–801.
Bitman, R., Sakaki, T., Tsuji, A., Devaux, P. F. and Ohnishi, S. I. (1984).Biochim. Biophys. Acta 769:85–95.
Seigneuret, M., Favre, E., Morrot, G. and Devaux, P. F. (1985).Biochim. Biophys. Acta 813:174–182.
Taylor, M. and Smith, I. C. P. (1983).Biochim. Biophys. Acta 733:256–263.
Glaser, R., Fujii, T., Müller, P., Tamura, E. and Herrmann, A. (1987).Biochim. Biomed. Acta, in press.
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Herrmann, A., Müller, P. Ionic strength-dependent alterations of membrane structure of red blood cells. Biosci Rep 6, 1007–1015 (1986). https://doi.org/10.1007/BF01114978
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DOI: https://doi.org/10.1007/BF01114978