Abstract
The sodium contents of β-cell-rich pancreatic islets fromob/ob-mice were measured with an integrating flame photometer. After washing to an apparent steady state with different types of ice-cold media, islets incubated in the absence of glucose contained 79–108 mmol sodium kg−1 dry weight. Exposure to glucose resulted in 25 % reduction of the islet content of sodium. This effect became manifest in the presence of 5 mM glucose, there being no additional reduction with a further increase of glucose to 20 mM. Depression of Na+ activity may partially explain why glucose, under certain conditions, can lower cytoplasmic Ca2+ and even inhibit insulin release.
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References
Atwater, I., Ribalet, B., and Rojas, E. (1978).J. Physiol. 278:117–139.
Bergsten, P. and Hellman, B. (1984).Biochem. Biophys. Res. Commun. 125:875–881.
Biden, T. J., Janjic, D., and Wollheim, C. B. (1986).Am. J. Physiol. 250:C207-C213.
Brown, A. M. and Kunze, D. L. (1974). In:Ion-Selective Microelectrodes (H. J. Brennan, and N. C. Herbert, eds), Plenum Press, New York, pp. 57–73.
Donatsch, P., Lowe, D. A., Richardson, B. P., and Taylor, P. (1977).J. Physiol. 267:357–376.
Gagerman, E., Sehlin, J., and Täljedal, I. B. (1980).J. Physiol. 300:505–513.
Hahn, H. J., Hellman, B., Lernmark, Å., Sehlin, J., and Täljedal, I. B. (1974).J. Biol. Chem. 249:5275–5284.
Hales, C. N. and Milner, R. D. G. (1968).J. Physiol. 194:725–743.
Hellman, B. (1970).Diabetologia 6:110–120.
Hellman, B. (1975).Endocrinology 97:392–398.
Hellman, B. (1985).Diabetologia 28:494–501.
Hellman, B. (1986).Diab./Metab. Rev. 2:215–241.
Hellman, B., and Gylfe, E. (1986). In:Calcium and Cell Function (W. Y. Cheung, ed.), Vol. 6; Academic Press, New York, pp. 253–326.
Hellman, B., Sehlin, J., and Täljedal, I. B. (1971).Diabetologia 7:256–265.
Hellman, B., Andersson, T., Berggren, P.-O., and Rorsman, P. (1980).Biochem. Med. 24:143–152.
Henquin, J. C. and Meissner, H. P. (1984).Experientia 40:1043–1052.
Herchuelz, A. and Malaisse, W. J. (1980).J. Physiol. 302:263–280.
Kalkhoff, R. K., and Siegesmund, K. A. (1981).J. Clin. Invest. 68:517–524.
Kawazu, S., Boschero, A. C., Delcroix, C., and Malaisse, W. J. (1978).Pflügers Arch. 375:197–206.
Lindh, U., Juntti-Berggren, L., Berggren, P.-O., and Hellman, B. (1985).Biomed. Biophys. Acta 44:55–62.
Lowe, D. A., Richardson, B. P., Taylor, P., and Donatsch, P. (1976).Nature (Lond.) 260:337–338.
MacDonald, M. J. (1984).Biochem. Int. 8:771–778.
Meissner, H. P., Henquin, J. C., and Preissler, M. (1978).FEBS Lett. 94:87–89.
Öberg, P. Å., Ulfendahl, H. R., and Wallin, B. G. (1967).Anal. Biochem. 18:543–558.
Pace, C. S. (1979).Am. J. Physiol. 237:E130-E135.
Prentki, M. and Wollheim, C. B. (1984).Experientia 40:1052–1060.
Rorsman, P., Abrahamsson, H., Gylfe, E., and Hellman, B. (1984).FEBS Lett. 170: 196–200.
Sehlin, J. and Täljedal, I.-B. (1974).FEBS Lett. 39:209–213.
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Wesslén, N., Bergsten, P. & Hellman, B. Glucose-induced reduction of the sodium content in β-cell-rich pancreatic islets. Biosci Rep 6, 967–972 (1986). https://doi.org/10.1007/BF01114973
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DOI: https://doi.org/10.1007/BF01114973