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DNA sequences which influence the selection and efficient utilisation of transcriptional start sites of a eukaryotic gene by RNA polymerase IIin vitro andin vivo

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Abstract

Experiments are described which probe the relationship between three sequence elements which make up the eukaryotic RNA polymerase II promoter. A cloned eukaryotic gene, from which the TATA-box and 400 base pairs of Y-flanking sequence has been deleted, is still transcriptionally activein vivo (following its transfection into cultured mammalian cells) andin vitro. Deletion has appropriately positioned a cluster of five TATA box-like sequences upstream from multiple potential cap sites. Which cap sites are actually used can be predicted from the DNA sequence of TATA box-like sequences and their spatial relationship with respect to possible transcriptional start sites, although there appears to be some difference in cap site utilisationin vitro andin vivo. Data suggest that deletion has also removed “upstream” sequences which affect promoter function.

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Authors and Affiliations

  1. Department of Biochemistry, University College London, Gower Street, WC1E 6BT, London, UK

    Balazs J. Kovacs & Peter H. W. Butterworth

Authors
  1. Balazs J. Kovacs
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  2. Peter H. W. Butterworth
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Kovacs, B.J., Butterworth, P.H.W. DNA sequences which influence the selection and efficient utilisation of transcriptional start sites of a eukaryotic gene by RNA polymerase IIin vitro andin vivo . Biosci Rep 6, 937–944 (1986). https://doi.org/10.1007/BF01114969

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  • DOI: https://doi.org/10.1007/BF01114969

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