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Enzyme linked immunosorbent assay (ELISA) for the determination of protein-A

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Abstract

A competitive ELISA for the determination of protein-A concentration in culture supernatants is described. The sensitivity of the assay is 50 ng/ml and it may be used to determine concentrations of protein-A up to 1000 ng/ml. The assay is specific, rapid and suitable for routine screening of protein-A producing microorganisms.

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Authors and Affiliations

  1. IIRS, Biochemicals Unit, University College, Galway, Ireland

    P. J. Considine

  2. Noctech SBD Centre, University College, Galway, Ireland

    P. Duggan & A. Eadie

Authors
  1. P. J. Considine
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  2. P. Duggan
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  3. A. Eadie
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Considine, P.J., Duggan, P. & Eadie, A. Enzyme linked immunosorbent assay (ELISA) for the determination of protein-A. Biosci Rep 6, 933–936 (1986). https://doi.org/10.1007/BF01114968

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  • DOI: https://doi.org/10.1007/BF01114968

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