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Viroid RNA is accepted as a template for in vitro transcription by DNA-dependent DNA polymerase I and RNA polymerase fromEscherichia coli

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Bioscience Reports

Abstract

The RNA genome of potato spindle tuber viroid (PSTV) is transcribed in vitro into complementary DNA and RNA by DNA-dependent DNA polymerase I and RNA polymerase, respectively, fromEscherichia coli . In vitro synthesis of complementary RNA produces distinct transcripts larger than unit length thus reflecting the in vivo mechanism of viroid replication. The influence of varying experimental conditions on the transcription process is studied; actinomycin D is found to drastically reduce complementary RNA synthesis from the PSTV RNA template by RNA polymerase.

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Authors and Affiliations

  1. Institut für Virologie, Justus-Liebig-Universität Giessen, D-6300, Giessen, FRG

    Wolfgang Rohde

  2. Max-Planck-Institut für Biochemie, D-8033, Martinsried, FRG

    Hans-Richard Rackwitz & Heinz L. Sänger

  3. Arbeitsgruppe Pflanzenvirologie, Justus-Liebig-Universität Giessen, D-6300, Giessen, FRG

    Frank Boege & Heinz L. Sänger

Authors
  1. Wolfgang Rohde
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  2. Hans-Richard Rackwitz
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  3. Frank Boege
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  4. Heinz L. Sänger
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Rohde, W., Rackwitz, HR., Boege, F. et al. Viroid RNA is accepted as a template for in vitro transcription by DNA-dependent DNA polymerase I and RNA polymerase fromEscherichia coli . Biosci Rep 2, 929–939 (1982). https://doi.org/10.1007/BF01114900

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  • DOI: https://doi.org/10.1007/BF01114900

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