Skip to main content
SpringerLink
Log in

Lanthanide-ion transport across phospholipid vesicular membranes: a comparison of alamethicin 30 and A23187 using1H-NMR spectroscopy

  • Published:
Bioscience Reports

Abstract

The kinetics of Pr3+ transport by the ionophores alamethicin 30 and A23187 across unilamellar phospho-lipid vesicular membranes has been compared by following the time-dependent changes in the1H-NMR spectrum of the vesicles. The measured rates of transport allow stoichiometries of the transporting species to be deduced which are consistent with channel- and carrier-mediated mechanisms respectively. The method provides a useful complement to planar bilayer conductivity studies of these systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hokin LE (1981) J. Membrane Biol.60, 77–93.

    Google Scholar 

  2. Ting TZ, Hagan PS, Chan SI, Doll JO & Springer CS Jr (1981) Biophys. J.34, 189–216.

    PubMed  Google Scholar 

  3. Pike MM, Simon SR, Balschi JA & Springer CS Jr (1982) Proc. Natl. Acad. Sci. U.S.A.79, 810–814.

    PubMed  Google Scholar 

  4. Hunt GRA (1975) FEBS Lett.58, 194–196.

    PubMed  Google Scholar 

  5. Hunt GRA & Tipping LRH (1978) Biophysical Chem.8, 341–355.

    Google Scholar 

  6. Hunt GRA & Jawahalal K (1980) Biochim. Biophys. Acta601, 678–684.

    PubMed  Google Scholar 

  7. Hunt GRA (1980) FEBS Lett.119, 132–136.

    PubMed  Google Scholar 

  8. Hunt GRA (1980) Phys. Chem. Lipids,27, 353–364.

    Google Scholar 

  9. Gögelein H, de Smedt, H., Van Driessche, W & Borghgraef R (1981) Biochim. Biophys. Acta640, 185–194.

    PubMed  Google Scholar 

  10. Lau ALY & Chan SI (1974) Biochemistry13, 4942–4948.

    PubMed  Google Scholar 

  11. Lau ALY & Chan SI (1975) Proc. Natl. Acad. Sci. U.S.A.72, 2170–2174.

    PubMed  Google Scholar 

  12. Lau ALY & Chan SI (1976) Biochemistry15, 2551–2555.

    PubMed  Google Scholar 

  13. Hunt GRA & Tipping LRH (1978) Biochim. Biophys. Acta507, 242–261.

    PubMed  Google Scholar 

  14. Bergelson LD (1978) inMethods in Membrane Biology (Korn E, ed), vol. 9, pp 275–335.

  15. Hutton WC, Yeagle PL & Martin RB (1977) Chem. Phys. Lipids19, 255–265.

    PubMed  Google Scholar 

  16. Liao MJ & Prestegard JH (1979) Biochim. Biophys. Acta550, 157–173.

    PubMed  Google Scholar 

  17. Chaney MO, Jones ND & Debono M (1976) J. Antibiotics29, 422.

    Google Scholar 

  18. Hughes MN (1981)The Inorganic Chemistry of Biological Processes (2nd ed), p 273, Wiley.

  19. dos Remedios CG (1981) Cell Calcium2, 29–51.

    Google Scholar 

  20. Bamberg E & Lauger P (1977) J. Memb. Biol.35, 351–375.

    Google Scholar 

  21. Haydon DA & Hladky SB (1972) Quart. Rev. Biophys.5, 187–282.

    Google Scholar 

  22. Cherry RJ, Chapman D & Graham DE (1972) J. Membrane Biol. 7, 325–344.

    Google Scholar 

  23. Tosteson MT & Tosteson DC (1981) Biophys. J.36, 109–116.

    PubMed  Google Scholar 

  24. Baumann G & Mueller P (1974) J. Supramolec. Struct.2, 583–557.

    Google Scholar 

  25. Boheim G & Kolb H-A (1978) J. Membrane Biol.38, 99–150.

    Google Scholar 

  26. Fringeli UP & Fringeli M (1979) Proc. Natl. Acad. Sci. U.S.A.76, 3852–3856.

    PubMed  Google Scholar 

  27. Fringeli UP (1980) J. Membrane Biol.54, 203–212.

    Google Scholar 

  28. Schindler H (1979) FEBS Lett.104, 157.

    PubMed  Google Scholar 

  29. Melling J & McMullen AI (1975) ISC-IAMS Proceedings, Science Council of Japan5, 446–452.

    Google Scholar 

  30. Pierce HD Jr, Unran AM & Oehlschlager AC (1978) Can. J. Biochem.56, 801–807.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Department of Science, The Polytechnic of Wales, CF37 1DL, Pontypridd, UK

    G. R. A. Hunt & I. C. Jones

Authors
  1. G. R. A. Hunt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. C. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hunt, G.R.A., Jones, I.C. Lanthanide-ion transport across phospholipid vesicular membranes: a comparison of alamethicin 30 and A23187 using1H-NMR spectroscopy. Biosci Rep 2, 921–928 (1982). https://doi.org/10.1007/BF01114899

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01114899

Keywords

Search

Navigation