Abstract
The dideoxy sequencing technique has been applied to the direct sequencing of large double-stranded DNA molecules with a small single-stranded primer. For instance, the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure proves useful in the sequence analysis of a large number of different mutations in a particular region and in the analysis of eukaryotic DNA cloned in plasmids, phages, and cosmids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sanger F, Nicklen S & Coulson AR (1977) Proc. Natl. Acad. Sci. U.S.A.74, 5463–5467.
Sanger F, Coulson AR, Barrell BG, Smith AJH & Roe BA (1980) J. Mol. Biol.143, 161–178.
Messing J, Crea R & Seeburg PH (1981) Nucl. Acids Res.9, 309–321.
Hong GF (1981) Biosci. Rep.1, 243–252.
Smith M, Leung DW, Gillam S & Astell CR (1979) Cell16, 753–761.
Wallace RB, Johnson MJ, Suggs SV, Miyoshi K, Bhatt R & Itakura K (1981) Gene16, 21–26.
Wallace RB, Schold M, Johnson MJ, Dembek P & Itakura K (1981) Nucl. Acids Res.9, 3647–3656.
Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB (1982) J. Mol. Biol., in press.
Gait MJ, Matthes HWD, Singh M, Sproat BS & Titmas RC (1982) Nucl. Acids Res., in press.
Staden R (1977) Nucl. Acids Res.4, 4037–4051.
Rosenberg M, Court D, Shimatake H, Brady C & Wulff DL (1978) Nature272, 414–423.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hong, G.F. Sequencing of large double-stranded DNA using the dideoxy sequencing technique. Biosci Rep 2, 907–912 (1982). https://doi.org/10.1007/BF01114897
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01114897