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Glutamine uptake by rat renal basolateral membrane vesicles

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Bioscience Reports

Abstract

The presence of a sodium-dependent, saturable uptake process is described in basolateral membranes of rat renal cortex for L-glutamine. Concentration-dependence studies indicate the presence of multiple transport systems withK m 1 of 0.032 mM and V1 of 0.028 nmol/mg of protein per min, andK m 2 of 17.6 mM and V2 of 17.6 nmol/mg of protein per min. Lysine completely inhibits the high-affinity, low-capacityK m system and partially inhibits the low-affinity, high-capacity system. Cystine and other dibasic amino acids also affect glutamine uptake.

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Author notes

  1. Hanna Wald

    Present address: Nephrological Service, Hadassah University Hospital, Ein Kerem, Jerusalem, Israel

Authors and Affiliations

  1. Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Blvd., 19104, Philadelphia, PA, USA

    Robert A. Reynolds, Hanna Wald & Stanton Segal

  2. Departments of Pediatrics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    Robert A. Reynolds, Hanna Wald & Stanton Segal

Authors
  1. Robert A. Reynolds
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  2. Hanna Wald
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  3. Stanton Segal
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Reynolds, R.A., Wald, H. & Segal, S. Glutamine uptake by rat renal basolateral membrane vesicles. Biosci Rep 2, 883–890 (1982). https://doi.org/10.1007/BF01114894

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  • DOI: https://doi.org/10.1007/BF01114894

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