Bioscience Reports

, Volume 9, Issue 6, pp 737–745 | Cite as

Export of proteins across membranes: The helix reversion hypothesis

  • U. P. Shinde
  • T. N. Guru Row
  • Y. R. Mawal
Short Papers
  • 3 Downloads

Abstract

A model is presented which explains the biological role of the leader peptide in protein export. Along the lines of this model, the conformational changes of a protein with environment serves as a general mechanism for translocation. The leader peptide in the cytoplasm takes a hairpin like conformation which reverts to an extended helix upon integration into the membrane. The essential features of this model are in accord with recent results of protein export.

Key Words

protein export conformational changes leader peptides hairpin helix 

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References

  1. 1.
    Randall, L. L., Hardy, S. J. S. and Thom, J. R. (1987)Ann. Rev. Microbiol. 41:507–541.Google Scholar
  2. 2.
    Von Heijne, G. (1988)Biochem. Biophys. Acta. 947:307–333.Google Scholar
  3. 3.
    Engelman, D. M. and Steitz, T. A. (1981)Cell 23:411–422.Google Scholar
  4. 4.
    Von Heijne, G. and Blomberg, C. (1979)Eur. J. Biochem. 97:175–181.Google Scholar
  5. 5.
    Blobel, G. and Dobberstein, B. (1975)J. Cell Biol. 67:835–851.Google Scholar
  6. 6.
    Briggs, M. and Gierasch, L. (1984)Biochemistry 23:3111–3114.Google Scholar
  7. 7.
    Briggs, M., Gierasch, L., Zlotnick, A., Lear, J. and DeGrado, W. (1985)Science 228:1096–1099.Google Scholar
  8. 8.
    Park, S., Liu, G., Topping, T. B., Cover, W. H. and Randall, L. L. (1988)Science 269:1033–1035.Google Scholar
  9. 9.
    Chou, P. Y. and Fasman, G. D. (1973)J. Mol. Biol. 74:263–281.Google Scholar
  10. 10.
    Bychkova, V. E., Pain, R. H. and Ptitsyn, O. B. (1988)FEBS Lett. 238:231–234.Google Scholar
  11. 11.
    Kaiser, E. T. and Kerdy, F. J. (1984)Science 223:249–255.Google Scholar
  12. 12.
    Walter, P., Ibrahimi, I. and Blobel, G. (1981)91:545–550.Google Scholar
  13. 13.
    Bernbeu, C. and Lake, J. A. (1982)Proc. Natl. Acad. Sci. USA,79:3111–3115.Google Scholar
  14. 14.
    Gilmore, R. and Blobel, G. (1982)J. Cell Biol. 96:470–477.Google Scholar
  15. 15.
    Walter, P., Ibrahim, I. and Blobel, G. (1981)J. Cell Biol. 91:551–556.Google Scholar
  16. 16.
    Walter, P. and Blobel, G. (1981)J. Cell Biol. 91:551–556.Google Scholar
  17. 17.
    Walter, P. and Blobel, G. (1981)J. Cell Biol. 91:557–561.Google Scholar
  18. 18.
    Rothman, J. E. and Lodish, H. E. (1977)Nature,269:575–580.Google Scholar
  19. 19.
    Andrews, D. W., Walter, P. and Ottensmeyer, F. P. (1985)Proc. Natl. Acad. Sci. USA,82:785–789.Google Scholar
  20. 20.
    Singer, S. J., Maher, P. A. and Yaffe, M. P. (1987)Proc. Natl. Acad. Sci. USA,84:1015–1019.Google Scholar
  21. 21.
    Rapoport, T. A., Heinrich, R., Walter, P. and Schulmeister, T. (1987)J. Mol. Biol. 195:621–636.Google Scholar
  22. 22.
    Ainger, K. J. and Meyer, D. I. (1986)EMBO J. 5:951–955.Google Scholar
  23. 23.
    Roitsch, T. and Lehle, L. (1988)Eur. J. Biochem. 174:699–705.Google Scholar
  24. 24.
    Kaiser, E. T. (1986) in: “Protein Engineering Applications in Science, Medicine and Industry”, (Inouye, M. and Sarma, R. eds.) Academic Press Inc. (London) Ltd. pp. 71–79.Google Scholar
  25. 25.
    Waxman, L and Goldberg, L. A. (1986)Science,232:500–503.Google Scholar
  26. 26.
    Rosenblatt, M., Beaudette, N. V. and Fasman, G. D. (1980)Proc. Natl. Acad. Sci. USA,77:3983–3987.Google Scholar
  27. 27.
    Briggs, M., Cornell, D., Dluhy, R. and Gierasch, L. (1986)Science 233:206–208.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • U. P. Shinde
    • 1
  • T. N. Guru Row
    • 2
  • Y. R. Mawal
    • 3
  1. 1.Division of Chemical EngineeringNational Chemical LaboratoryPuneIndia
  2. 2.Division of Physical ChemistryIndian Institute of ScienceBangalore
  3. 3.Division of Biochemical Sciences, Division of Chemical EngineeringNational Chemical LaboratoryPuneIndia

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