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Imbalance of CD4+CD29+ and CD4+CD45RA+ T-helper cell subsets in peripheral blood of patients with severe atopic dermatitis

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Abstract

To determine the proportion of T-helper cell subsets in the peripheral blood we studied 16 patients with mild, moderate and severe atopic dermatitis. Lymphocytes were isolated from heparinized peripheral blood and analysed by two-colour flow cytometry. Patients with severe atopic dermatitis had a decreased CD4+CD29+∶CD4+CD45RA+ ratio (p<0.01). We found a decreased absolute number of CD4+CD29+ cells (p<0.05) and an increased absolute number of CD4+CD45RA+ cells (p<0.05) in the peripheral blood. No significant changes in the CD4+CD29+∶CD4+CD45RA+ ratio were found in the peripheral blood of patients with clinically mild or moderate atopic dermatitis.

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References

  1. Aurich G, Göring HD, Kramer H, Schütt C, Schwenke H (1979) Empfehlungen zu einer klinischen Standardisierung des LTT. Allerg Immunol (Leipz) 25: 3–9

    Google Scholar 

  2. Beverly PC (1987) Human T cell subsets. Immunol Lett 263

  3. Bos JD, Zonneveld I, Das PK, Krieg SR, Van der Loos CM, Kapsenberg ML (1987) The skin immune system (SIS): distribution and immunophenotype of lymphocyte subpopulations in normal human skin. J Invest Dermatol 111: 569–573

    Google Scholar 

  4. Bos JD, Hagenaars C, Das PK, Krieg SR, Voorn WJ, Kapsenberg ML (1989) Predominance of “memory” T cells (CD4+, CDw29+) over “naive” T cells (CD4+CD45R+) in both normal and diseased human skin. Arch Dermatol Res 281: 24–30

    Google Scholar 

  5. Bruijnzeel-Koomen CAFM, Mudde GC, Bruijnzeel PLB (1989) New aspects in the pathogenesis of atopic dermatitis. Acta Derm Venereol Suppl (Stockh) 144: 58–63

    Google Scholar 

  6. Burgard J, Mielke V, Leimenstoll G, Sterry W (1991) CD4+ memory T cells in peripheral blood are not decreased in patients with atopic dermatitis. Dermatologica 182: 85–88

    Google Scholar 

  7. Franco E, Kawa-Ha K, Doi S, Yumura K, Murata M, Ishihara S, Tawa A, Yabuuchi H (1987) Remarkable depression of CD4+2H4+ T cells in severe chronic active Epstein-Barr virus infection. Scan J Immunol 26: 769–773

    Google Scholar 

  8. Hanifin JM, Rajka G (1980) Diagnostic features of atopic dermatitis. Acta Derm Venereol Suppl (Stockh) 92: 44–47

    Google Scholar 

  9. Kapsenberg ML, Wierenga EA, Bos JD, Jansen HM (1991) Functional subsets of allergen-reactive human CD4+ T cells. Immunol Today 12: 392–395

    Google Scholar 

  10. Mackay CR (1991) T-cell memory: the connection between function, phenotype and migration pathways. Immunol Today 12: 189–192

    Google Scholar 

  11. Morimoto C, Letvin NL, Boyd AW, Hagan M, Brown HM, Kornacki MM, Schlossman SF (1985) The isolation and characterization of the human helper inducer T cell subset. J Immunol 134: 4762–3769

    Google Scholar 

  12. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF (1985) The isolation and characterization of the human suppressor inducer T cell subset. J Immunol 134: 1508–1515

    Google Scholar 

  13. Morimoto C, Hafler DA, Weiner HL, Letvin H, Hagan M, Daley J, Schlossman SF (1987) Selective loss of the suppressor-inducer T cell subset in progressive multiple sclerosis. N Engl J Med 316: 67

    Google Scholar 

  14. Mosmann TR, Cherwinski H, Bond MW et al. (1986) Two types of murine helper T-cell clones. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 136: 2348–2357

    Google Scholar 

  15. Mylvaganam R, Ahn YS, Sprinz PG, Garcia RO, Harrington WY (1989) Sex difference in the CD4+CD45R+ T lymphocyte in normal individuals and its selective decrease in women with idiopathic thrombocytopenic purpura. Clin Immunol Immunopathol 52: 473–485

    Google Scholar 

  16. Pearce EJ, Caspar P, Crzych JM, Lewis FA, Sher A (1991) Downregulation of Th1 cytokine production accompanies induction of Th2 response by a parasitic helminthSchistosoma mansoni. J Exp Med 173: 159–166

    Google Scholar 

  17. Ramb-Lindhauer C, Feldmann A, Rotte M, Neumann C (1991) Characterization of grass pollen reactive T-cell lines derived from lesional atopic skin. Arch Dermatol Res 283: 71–76

    Google Scholar 

  18. Romagnani S (1991) Human Th1 and Th2 subsets. Doubt no more. Immunol Today 12: 256–257

    Google Scholar 

  19. Romagnani S, Del Petre GF, Maggi E, Parronchi P, Tiri A, Macchia D, Simonelli C, Biswas P, De Carli M, Ricci M (1989) T-cell influence on IgE synthesis. Allergologie 12: 50–53

    Google Scholar 

  20. Rose LM, Ginsberg AH, Rothstein TL, Ledbetter JA, Clark EA (1985) Selective loss of a subset of T helper cells in active multiple sclerosis. Proc Natl Acad Sci USA 82: 7389

    Google Scholar 

  21. Sanders ME, Makgoba MW, Sharrow SO, Stephany D, Springer TA, Young HA, Shaw S (1988) Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29 and Pgp-1) and have enhanced IFN-gamma production. J Immunol 140: 1401–1409

    Google Scholar 

  22. Sanders ME, Makgoba MW, Shaws (1988) Human naive and memory T cells: reinterpretation of helper-inducer and suppressor-inducer subsets. Immunol Today 9: 195–199

    Google Scholar 

  23. Schauer U, Jung T, Heymanns J, Rieger CHL (1990) Imbalance of CD4+CD45R+ and CD4+CD29+ T helper cell subsets in patients with atopic diseases. Clin Exp Immunol 82: 1–5

    Google Scholar 

  24. Ternowitz T, Herlin T (1986) Defective monocyte and p.m.n. leukocyte chemotaxis and clinical characteristics in atopic dermatitis. Arch Dermatol Res 278: 454–459

    Google Scholar 

  25. Thestrup-Pedersen K (1989) Immunology of atopic dermatitis. Acta Derm Venereol (Stockh) 69 [Suppl 151]: 77–83

    Google Scholar 

  26. Tilmant L, Dessaint JP, Tsicopoulos A, Tonnel AB, Capron A (1989) Concomitant augmentation of CD4+CD45R+ suppressor/inducer subsets and diminution of CD4+CDw29+ helper/inducer subset during rush hyposensitization in hymenoptera venom allergy. Clin Exp Immunol 76: 13–18

    Google Scholar 

  27. Umetsu DT, Dekruyff RH (1988) Induction of IgE synthesis by human inducer T cell clones secreting interleukin 4 (BSF-1). J Allergy Clin Immunol 80: 303

    Google Scholar 

  28. Van der Heijden FL, Wierenga EA, Bos JD, Kapsenberg L (1991) High frequency of IL-4-producing CD4+ allergen-specific T lymphocytes in atopic dermatitis lesional skin. J Invest Dermatol 97: 389–394

    Google Scholar 

  29. Verwilghen J, Vertessen S, Stevens EAM, Dequeker J, Ceuppens JL (1990) Depressed T-cell reactivity to recall antigens in rheumatoid arthritis. J Clin Immunol 10: 90–98

    Google Scholar 

  30. Vuillier F, Lapresle C, Dighiero G (1988) Comparative analysis of CD4-4B4 and CD4-2Ha lymphocyte subpopulations in HIV negative homosexual, HIV seropositive and healthy subjects. Clin Exp Immunol 71: 8–12

    Google Scholar 

  31. Waldorf HA, Walsh LJ, Schechter NM, Murphy GF (1991) Early cellular events in evolving cutaneous delayed hypersensitivity in humans. Am J Pathol 138: 477–486

    Google Scholar 

  32. Wierenga E, Snoek M, De Groot C, Chretien I, Bos JD, Jansen HM, Kapsenberg ML (1990) Evidence for compartmentalization of functional subsets of CD4+ lymphocytes in atopic patients. J Immunol 144: 4651–4656

    Google Scholar 

  33. Wüthrich B, Joller-Jemelka HJ, Grob PJ (1989) Soluble IL-2 receptors in atopic dermatitis — relationship to severity of cutaneous involvement and course of disease as well as classification into an “exctrinsic” and an “intrinsic” type of atopic dermatitis. Allergologie 12: 98

    Google Scholar 

  34. Zachary CB, Allen MH, MacDonald DM (1985) In situ quantification of T-lymphocyte subsets and Langerhans cells in the inflammatory infiltrate of atopic eczema. Br J Dermatol 112: 149–156

    Google Scholar 

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Authors and Affiliations

  1. Klinik und Poliklinik für Hautkrankheiten der Medizinischen Hochschule, ArnstÄdter Strasse 34, O-5082, Erfurt, Germany

    K. Jung, V. Klimmek & H. Schubert

  2. Abteilung für Immunologie, Medizinische Hochschule, Erfurt, Germany

    A. Bittrich & V. Klimmek

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Jung, K., Bittrich, A., Klimmek, V. et al. Imbalance of CD4+CD29+ and CD4+CD45RA+ T-helper cell subsets in peripheral blood of patients with severe atopic dermatitis. Arch Dermatol Res 285, 135–139 (1993). https://doi.org/10.1007/BF01112915

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  • DOI: https://doi.org/10.1007/BF01112915

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