Archives of Dermatological Research

, Volume 283, Issue 4, pp 219–223 | Cite as

Stratum corneum lipid abnormalities in atopic dermatitis

  • A. Yamamoto
  • S. Serizawa
  • M. Ito
  • Y. Sato
Original Contributions


Patients with atopic dermatitis (AD) often present with a dry skin. To clarify the relationship between dry skin and lipid abnormalities within stratum corneum, stratum corneum lipids were collected from six AD patients aged 15 to 25 years and from sex- and age-matched controls. All major stratum corneum lipid classes were separated and quantitated by high-performance thin-layer chromatography/photodensitometry. Six ceramide fractions were also isolated and quantitated by thin-layer chromatography/photodensitometry. Esterified fatty acids of both ceramide 1 (acylceramides) and wax esters were analysed by capillary gas chromatography. The relative amounts of all the stratum corneum lipid classes including squalene, cholesterol esters, wax esters, triglycerides, free fatty acids, cholesterol, ceramides, cholesterol sulphate and phospholipids did not differ statistically between AD patients and controls. However, a significant decrease in proportion of ceramide 1, which is believed to be a carrier of linoleate responsible for a water-barrier function, and increased levels of esterified C18∶1 fatty acids (oleate) of ceramide 1 were observed in AD patients. On the other hand, the fatty acid compositions as well as the proportions of C16∶1 straight-chain component in sebum wax esters of AD patients were very similar to those of controls. These results suggest that a significantly reduced amount and/or structural alterations of ceramide 1 deriving from epidermal keratinocytes may be responsible for the impaired water-barrier function of the skin in AD.

Key words

Ceramide 1 Oleate Atopic dermatitis Water-barrier function 


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  1. 1.
    Abe T, Ohkido M, Yamamoto K (1978) Studies on skin surface barrier functions: skin surface lipids and transepidermal water loss in atopic skin during childhood. J Dermatol (Tokyo) 5:223–229Google Scholar
  2. 2.
    Abraham W, Wertz PW, Downing DT (1985) Linoleate-rich acylglucosylceramides of pig epidermis: structure determination by proton magnetic resonance. J Lipid Res 26:761–765PubMedGoogle Scholar
  3. 3.
    Elias PM (1983) Epidermal lipids, barrier function and desquamation. J Invest Dermatol 80:44s-49sPubMedGoogle Scholar
  4. 4.
    Elias PM, Friend DS (1975) The permeability barrier in mammalian epidermis. J Cell Biol 65:180–191PubMedGoogle Scholar
  5. 5.
    Elias PM, Brown BE, Ziboh VA (1980) The permeability barrier in essential fatty acid deficiency: evidence for a direct role for linoleic acid in barrier function. J Invest Dermatol 74:230–233PubMedGoogle Scholar
  6. 6.
    Gray GM, White RJ, Major JR (1978) 1-(3′-O-Acyl)-Β-glucosyl-N-dihydroxypentatriacontadienoyl-sphingosine, a major component of the glucosylceramides of pig and human epidermis. Biochim Biophys Acta 528:127–137PubMedGoogle Scholar
  7. 7.
    Hanifin JM, Rajka G (1980) Diagnostic features of atopic dermatitis. Acta Derm Venereol Suppl (Stockh) 92:44–47Google Scholar
  8. 8.
    Imokawa G, Hattori M (1985) A possible function of structural lipids in the water-holding properties of the stratum corneum. J Invest Dermatol 84:282–284PubMedGoogle Scholar
  9. 9.
    Imokawa G, Akasaki S, Hattori M, Yoshizuka N (1986) Selective recovery of deranged water-holding properties by stratum corneum lipids. J Invest Dermatol 87:758–761PubMedGoogle Scholar
  10. 10.
    Long SA, Wertz PW, Strauss JS, Downing DT (1985) Human stratum corneum polar lipids and desquamation. Arch Dermatol Res 277:284–287PubMedGoogle Scholar
  11. 11.
    Madison KC, Swartzendruber DC, Wertz PW, Downing DT (1987) Presence of intact intercellular lipid lamellae in the upper layers of the stratum corneum. J Invest Dermatol 88:714–718PubMedGoogle Scholar
  12. 12.
    Melnik B, Plewig B (1989) Ein neues Konzept zur Ätiopathogenese und PrÄvention der Atopie. Hautarzt 40:685–692PubMedGoogle Scholar
  13. 13.
    Melnik B, Hollmann J, Plewig G (1988) Decreased stratum corneum ceramides in atopic individuals: a pathobiochemical factor in xerosis? Br J Dermatol 118:547–549Google Scholar
  14. 14.
    Melnik BC, Hollman J, Erler E, Verhoeven B, Plewig G (1989) Microanalytical screening of all major stratum corneum lipids by sequential high-performance thin-layer chromatography. J Invest Dermatol 92:231–234PubMedGoogle Scholar
  15. 15.
    Perisho K, Wertz PW, Madison KC, Stewart ME, Downing DT (1988) Fatty acids of acylceramides from comedones and from the skin surface of acne patients and control subjects. J Invest Dermatol 90:350–353PubMedGoogle Scholar
  16. 16.
    Rajka G (1974) Transepidermal water loss on the hands in atopic dermatitits. Arch Dermatol Forsch 251:111–115PubMedGoogle Scholar
  17. 17.
    Stewart ME, Downing DT (1985) Measurement of sebum secretion rate in young children. J Invest Dermatol 84:59–61PubMedGoogle Scholar
  18. 18.
    Stewart ME, Quinn MA, Downing DT (1982) Variability in the fatty acid composition of wax esters from vernix caseosa and its possible relation to sebaceous gland activity. J Invest Dermatol 78:291–295PubMedGoogle Scholar
  19. 19.
    Stewart ME, Grahek MO, Cambier LS, Wertz PW, Downing DT (1986) Dilutional effect of increased sebaceous gland activity on the proportion of linoleic acid in sebaceous wax esters and in epidermal acylceramides. J Invest Dermatol 87:733–736PubMedGoogle Scholar
  20. 20.
    Stewart ME, Steele WA, Downing DT (1989) Changes in the relative amounts of endogenous and exogenous fatty acids in sebaceous lipids during early adolescence. J Invest Dermatol 92:371–378PubMedGoogle Scholar
  21. 21.
    Strauss JS, Pochi PE (1961) The quantitative gravimetric determination of sebum production. J Invest Dermatol 36:293–298Google Scholar
  22. 22.
    Werner Y, Lindberg M (1985) Transepidermal water loss in dry and clinically normal skin in patients with atopic dermatitis. Acta Derm Venereol (Stockh) 65:102–105Google Scholar
  23. 23.
    Werner Y, Lindberg M, Forslind B (1987) Membrane-coating granules in “dry” non-eczematous skin of patients with atopic dermatitis. Acta Derm Venereol (Stockh) 67:385–390Google Scholar
  24. 24.
    Wertz PW, Cho ES, Downing DT (1983) Effect of essential fatty acid deficiency on the epidermal sphingolipids of the rat. Biochim Biophys Acta 753:350–355PubMedGoogle Scholar
  25. 25.
    Wertz PW, Downing DT, Freinkel RK, Traczyk TN (1984) Sphingolipids of the stratum corneum and lamellar granules of fetal rat epidermis. J Invest Dermatol 83:193–195PubMedGoogle Scholar
  26. 26.
    Wertz PW, Miethke MC, Long SA, Strauss JS, Downing DT (1985) The composition of the ceramides from human stratum corneum and from comedones. J Invest Dermatol 84:410–412PubMedGoogle Scholar
  27. 27.
    Wertz PW, Downing DT (1983) Ceramides of pig epidermis: structure determination. J Lipid Res 24:759–765PubMedGoogle Scholar
  28. 28.
    Wertz PW, Downing DT (1983) Acylglucosylceramides of pig epidermis: structure determination. J Lipid Res 24:753–758PubMedGoogle Scholar
  29. 29.
    Wertz PW, Downing DT (1990) Metabolism of topically applied fatty acid methyl esters in BALB/C mouse epidermis. J Dermatol Sci 1:33–38PubMedGoogle Scholar
  30. 30.
    Yamamoto A, Serizawa S, Ito M, Sato Y (1987) Effect of aging on sebaceous gland activity and on the fatty acid composition of wax esters. J Invest Dermatol 89:507–512PubMedGoogle Scholar
  31. 31.
    Yamamoto A, Serizawa S, Ito M, Sato Y (1990) Fatty acid composition of sebum wax esters and urinary androgen levels in normal human individuals. J Dermatol Sci 1:269–276PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • A. Yamamoto
    • 1
  • S. Serizawa
    • 1
  • M. Ito
    • 1
  • Y. Sato
    • 1
  1. 1.Department of DermatologyNiigata University School of MedicineNiigataJapan

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