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Biological evaluation of pearl millet protein: effect of different treatments and storage

Abstract

Whole grain flour of one variety (HC-4) of pearl millet (Pennisetum americanum L. Leeke) after giving different treatments (defatting, butylated hydroxyanisole, ascorbic acid and thermal) was stored in earthen pots at prevailing room temperature (28–34°C) and relative humidity (70–80%) for 30 days. The flour samples were evaluated for protein quality using rats. Storage of flour for 30 days markedly reduced the protein quality. The values of protein efficiency ratio, true digestibility, biological value, net protein retention and net protein utilisation were significantly (P<0.05) higher in fresh and treated flour than untreated flour. Among treated flours, defatted flour showed best growth followed by butylated hydroxyanisole, thermal and ascorbic acid treated flour.

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References

  1. Burton GW, Wallace AT, Rachie KO (1972) Chemical composition and nutritive value of pearl millet grain. Crop Sci 12: 187–190

    Google Scholar 

  2. Carnovale E, Quaglia GB (1973) Influence of temperature and humidity controlled storage on chemical composition of milling products from millet. Am Technol Agric 22: 371–380

    Google Scholar 

  3. Lai CC, Variano-Marston E (1980) Changes in pearl millet meal during storage. Cereal Chem 57: 275–277

    Google Scholar 

  4. Chaudhary P, Kapoor AC (1984) Changes in the nutritive value of pearl millet flour during storage. J. Sci Food Agric 35: 1219–1224

    Google Scholar 

  5. Cheftal JC (1977) Chemical and Nutritional modifications of food proteins due to processing and storage. In: Whitaker JR, Tannen Beum ST (eds), Food Proteins, Westport, Connecticut: AVI Publishing Company Inc., pp. 401–445

    Google Scholar 

  6. Saio K (1980) Nutritive losses in storage and processing of legumes. In: Santos W, Lopes M, Barbosa JJ, Chaves D, Valente JC (eds) Nutrition and Food Science, New York: Plenum Press, pp. 421–450

    Google Scholar 

  7. Boora P, Kapoor AC (1985) Influence of storage on protein quality of pearl millet flour. J Sci Food Agric 36: 59–62

    Google Scholar 

  8. AOAC (1980) Official Methods of Analysis, Horwitz W (ed.). Washington, D.C.: Association of Official Analytical Chemists, pp. 211–214

    Google Scholar 

  9. Chapman DG, Castillo R, Campbell JA (1959) Evaluation of protein in foods. Can J Biochem Physiol 37: 679–686

    PubMed  Google Scholar 

  10. Miller GA, Lachance PA (1977) Techniques in rat bioassays. In: Bodwell CE (ed.), Evaluation of Proteins For Human. Westport, Connecticut: AVI Publishing Company Inc., pp. 156–159

    Google Scholar 

  11. Bender AE, Doel BH (1957) Biological evaluation of protein. A new aspect. Br J Nutr 11: 140–148

    PubMed  Google Scholar 

  12. Mitchell HH, Carma GG (1924) The biological value for the maintenance and growth of the proteins of whole wheat, eggs and pork. J Biol Chem 60: 613–620

    Google Scholar 

  13. Panse YG, Sukhatme PV (1961) Statistical Methods of Agricultural Workers. 2nd edn. New Delhi: Indian Council of Agricultural Research, 1287 pp.

    Google Scholar 

  14. Mohammed OE, Edrise BM, El-Bagouri AM, Morsey AA, Awad YL (1983) Studies on the biochemical and physiological changes inflicted by feeding rancid oils to chicken. J Egyp Vet Med Assoc 43: 405–417

    Google Scholar 

  15. Pushpamma S, Parrish DB, Deyo CW (1972) Improving protein quality of millet, sorghum and maize diets by supplementation. Nutr Rep Int 5: 93–100

    Google Scholar 

  16. Lohrey EE (1978) The influence of carbohydrate source on lipid oxidation in experimental diets and its effect on animal performance. Proceed Nutr Soc New Zealand 3: 129

    Google Scholar 

  17. Gray JI (1978) Measurement of lipid oxidation. A Rev J Am Oil Chem Soc 55: 539–546

    Google Scholar 

  18. Karel M (1973) Symposium protein interactions in biosystem. Protein lipid interactions. J Fd Sci 38: 756–763

    Google Scholar 

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Kapoor, R., Kapoor, A.C. Biological evaluation of pearl millet protein: effect of different treatments and storage. Plant Food Hum Nutr 40, 175–183 (1990). https://doi.org/10.1007/BF01104140

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  • DOI: https://doi.org/10.1007/BF01104140

Key words

  • protein quality
  • pearl millet
  • antioxidant
  • biological value
  • net protein utilisation