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A comparative study on cell wall antigens and cell surface hydrophobicity in clinical isolates ofCandida albicans

  • Human And Animal Mycology
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Abstract

Characterization of common cell surface-bound antigens inCandida albicans strains, particularly those expressed in the walls of mycelial cells might be useful in the diagnosis of systemic candidiasis. Hence, antigenic similarities among wall proteins and mannoproteins fromC. albicans clinical serotype A and B isolates, were studied using polyclonal (mPAbs) and monoclonal (MAb 4C12) antibodies raised against wall antigens from the mycelial form of a commonC. albicans serotype A laboratory strain (ATCC 26555). Zymolyase digestion of walls isolated from cells of the different strains studied grown at 37°C (germination conditions), released, in all cases, numerous protein and mannoprotein components larger than 100 kDa, along with a 33–34 kDa species. The pattern of major antigens exhibiting reactivity towards the mPAbs preparation was basically similar for all the serotype A and B isolates, though minor strain-specific bands were also observed. The immunodeterminant recognized by MAb 4C12 was found to be absent or present in very low amounts inC. albicans isolates other than the ATCC 26555 strain, yet high molecular weight species similar in size (e.g., 260 kDa) to the wall antigen against which MAb 4C12 was raised, were observed, particularly in wall digests from serotype A strains. Cell surface hydrophobicity, an apparently important virulence factor inC. albicans, of the cell population of each serotype B strain was lower than that of the corresponding serotype A counterparts, which is possibly due to the fact that the former strains exhibited a reduced ability to form mycelial filaments under the experimental conditions used.

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Abbreviations

CSH:

cell surface hydrophobicity

IIF:

indirect immunofluorescence

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Texas Tech University Health Science Center, 79430, Lubbock, Texas, USA

    José L. López-Ribot

  2. Unidad Departamental de Microbiología, Facultad de Medicina, Universitat de València, Spain

    David Navarro & José M. Nogueira

  3. Departamento de Microbiología, Facultad de Farmacia, Universitat de Valencia, Avda. Vicent Andrés Estellés, s/n., 46100, Burjassot, Valencia, Spain

    Pilar Sepúlveda, Manuel Casanova &  José P. Martinez

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  1. José L. López-Ribot
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  2. David Navarro
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  4. José M. Nogueira
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  5. Manuel Casanova
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  6. José P. Martinez
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López-Ribot, J.L., Navarro, D., Sepúlveda, P. et al. A comparative study on cell wall antigens and cell surface hydrophobicity in clinical isolates ofCandida albicans . Mycopathologia 127, 1–13 (1994). https://doi.org/10.1007/BF01104005

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  • DOI: https://doi.org/10.1007/BF01104005

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