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Clinico-pathological studies on experimental cryptococcal mastitis in goats

  • Human And Animal Mycology
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Unilateral intramammary inoculation of 10 goats withCryptococcus neoformans (2×106 yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and there was no dissemination of infection to the opposite uninfected udder halves as well as to other organs of the body. The experiment was continued for 40 days, with 2 animals each from the infected and control groups being killed on 5th, 10th, 20th, 30th and 40th day postinoculation (DPI). Initial enlargement of the infected udder halves was followed by marked decrease in size leading to very small, firm and nodular udder halves. After infection, there was also sharp fall in the milk yield. Cryptococcal organisms were demonstrated in the mastitic milk and udder impression smears with special stains.C. neoformans was reisolated from the milk of only infected udder halves up to 25th DPI. Microsopically, there was initially acute diffuse purulent mastitis which later on became chronic, characterised by marked infiltration of lymphocytes, macrophages, extensive fibrosis and development of multiple granulomas. The cryptococcal organisms could be demonstrated in the udder sections only up to 30th DPI. It is concluded that intramammary inoculation ofCryptococcus neoformans in goats leads to severe mastitis with sharp fall in milk yield.

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  1. Monga DP, Mahapatra LN, Kalra DS. Bovine mastitis caused byCryptococcus neoformans. Indian J Med Res 1970; 58: 1203–1205.

    PubMed  Google Scholar 

  2. Monga DP, Kalra DS. Prevalence of mycotic mastitis among animals in Hariana. Indian J Anim Sci 1971; 41: 813–816.

    Google Scholar 

  3. Pal M, Randhawa HS. Caprine mastitis due toCryptococcus neoformans. Sabouraudia 1976; 14: 261–263.

    PubMed  Google Scholar 

  4. Sharma SD, Rai P, Saxena SC. A survey of mycotic infections of udder in clinical and subclinical cases of mastitis in cows and buffaloes. Indian Vet J 1977; 54: 284–287.

    Google Scholar 

  5. Aljaburi AMI, Kalra DS. Cryptococcal mastitis in a goat: A case report. Haryana Veterinarian 1983; 22: 120–121.

    Google Scholar 

  6. Pal M, Mehrotra BS. Cryptococcal mastitis in dairy animals. Mykosen 1983; 26: 615–616.

    PubMed  Google Scholar 

  7. Sharma SD. Studies on bovine mastitis with special reference to mycotic infections of udder. Vet Res J 1983; 6: 105–106.

    Google Scholar 

  8. Aleandri M, Pin R. Isolation and identification ofCryptococcus neoformans from a cow with mastitis. Zooprofilassi 1964; 19: 15–18.

    Google Scholar 

  9. Mitroiu P, Toma C. Identification of Levuriform fungi from bovine mastitis. Inst Cerc Vet Bioprep Pasteur 1966; 4: 189.

    Google Scholar 

  10. El-Ghani MA, El-Sherif MT, El-Hamed SA. Prevalence of mycotic mastitis among sheep and goats in Egypt. J Egyptian Vet Med Assoc 1980; 38: 85–95.

    Google Scholar 

  11. El-Far F, Hammad HA, Refai M.Cryptococcus neoformans as a cause of bovine mastitis in Egypt. J Egyptian Vet Med Assoc 1987; 47: 203–208.

    Google Scholar 

  12. Luna LG. Manual of histological staining methods of the Armed Forces Institute of Pathology, 3rd ed. New York: McGraw Hill Book Company, 1968.

    Google Scholar 

  13. Kobayashi T, Kato O, Nakashima I, Kato N. Factors affecting experimental infection withCryptococcus neoformans in mice with special reference to an endotoxic substance ofC. neoformans. Mycopathologia 1975; 55: 17–22.

    PubMed  Google Scholar 

  14. Pounden WD, Amberson JM, Jaeger AB. A severe mastitis problem associated withCryptococcus neoformans in a large dairy herd. Am J Vet Res 1952; 13: 121–128.

    PubMed  Google Scholar 

  15. Farnsworth RJ. Significance of fungal mastitis. J Am Vet Med Assoc 1977; 170: 1173–1174.

    PubMed  Google Scholar 

  16. Nelli A, Podesta A, Socci A, Ruffo G. Experimental observations on some aetiopathogenic aspects of mycotic mastitis. 1970; 21: 143–150.

  17. Karaoni RM, Hall NK, Larsh HW. Role of macrophage in immunity and pathogenesis of experimental cryptococcosis induced by the air borne route. II. Phagocytosis and intracellular fate ofC. neoformans. Mykosen 1977; 20: 409–422.

    PubMed  Google Scholar 

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Singh, M., Gupta, P.P., Rana, J.S. et al. Clinico-pathological studies on experimental cryptococcal mastitis in goats. Mycopathologia 126, 147–155 (1994).

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