Abstract
Vast numbers of spores of the thermophilic actinomycetesExcellospora flexuosa,Thermomonospora alba,T. curvata andT. fusca were collected from the air in fermentation tunnels during the spawning of mushroom compost, i.e. over 109 CFU m−3 of air. Five different genera of fungi, namely,Aspergillus,Aureobasidium,Cladosporium,Penicillium andScytalidium, were found at only 103 CFU m−3 of air.Agaricus bisporus, used for spawning, was absent. Sera of 10 mushroom growers affected by Mushroom Worker's Lung (MWL) were tested by a qualitative dot-ELISA for antibodies against the spores of these micro-organisms. All 10 were positive for one or more of the actinomycetesE. flexuosa,T. alba,T. curvata andT. fusca. No antibodies were found againstStreptomyces thermovulgaris,Thermoactinomyces vulgaris andT. sacchari nor against the fungiAspergillus fumigatus,Penicillium brevicompactum,P. chrysogenum,Scytalidium thermophilum andTrichoderma viride. Sera of 11 of 14 workers engaged in routine spawning of compost in tunnels reacted positively with 1 or more of the actinomycetes. Their10log serum titres increased with the duration of employment to an upper limit of 2.5. The sera of 19 non-exposed individuals were negative. Because high numbers of spores ofE. flexuosa,T. alba,T. curvata andT. fusca were present in the air that was used for successful inhalation provocation of mushroom workers with MWL and because of the elevated serum titres of these workers, we presume these organisms to contribute in the occurrence of MWL.
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Derikx PJL, Op den Camp HJM, Van der Drift C, Van Griensven LJLD, Vogels GD. Biomass and biological activity during the production of compost used as a substrate in mushroom cultivation. Appl Environm Microbiol 1990; 56: 3029–34.
Gerrits JPG. Nutrition and compost. In: Van Griensven LJLD, ed. The cultivation of mushrooms. Rustington (UK): Darlington Mushroom Laboratories, 1988: 29–70.
Sinden JW, Hauser E. The nature of the composting process and its relation to short composting. Mushroom Sci 1953; 2: 123–31.
Crawford, DL. Biodegradation of agricultural and urban wastes. In: Goodfellow M, Williams ST, Mordarski M, eds. Actinomycetes in biotechnology. London: Academic Press, 1988: 433–59.
Straatsma G, Gerrits JPG, Augustijn MPAM, Op den Camp HJM, Vogels GD, Van Griensven LJLD. Population dynamics ofScytalidium thermophilum in mushroom compost and stimulatory effects on growth rate and yield ofAgaricus bisporus. J Gen Microbiol 1989; 35: 751–59.
Fergus CL. Thermophilic and thermotolerant moulds and actinomycetes of mushroom compost during peak heating. Mycologia 1964; 56: 267–84.
Lacey J. Allergy in mushroom workers. Lancet 1974(i): 366.
Moller BB, Halberg P, Gravesen S, Weeks B. Precipitating antibodies againstMicropolyspora faeni in sera from mushroom workers. Acta Allergol 1976; 31: 61–70.
Sakula A. Mushroom-Worker's Lung. Br Med J 1967; 3: 708–10.
Stolz JL, Arger PH, Benson JM. Mushroom worker's lung disease. Radiology 1976; 119: 61–63
Grant IWB. Extrinsic allergic alveolitis. In: Goetzl J, Kay AB, eds. Current perspectives in allergy. Edinburgh: Churchill, 1982: 78–92.
Roberts RC, Moore VL. Immunopathogenesis of hypersensitivity pneumonitis. Am Rev Respir Dis 1977; 116: 1075–90.
Van den Bogart HGG. De champignonkwekerslong: Een onderzoek naar voorkomen en etiologie in Nederland [Dissertation]. Nijmegen (The Netherlands): University of Nijmegen, 1990: 223p.
Kamm YJL, Folgering HTH, Van den Bogart HGG, Cox A. Provocation tests in extrinsic allergic alveolitis in mushroom workers. Neth J Med 1991; 38: 59–64.
Stedman OJ. A seven-day volumetric spore trap for use within buildings. Mycopathologia 1978; 66: 37–40.
Gams W, Van der Aa HA, Van der Plaats-Niterink AJ, Samson RA, Stalpers JA. CBS course of mycology. Baarn (The Netherlands): Centraal Bureau voor Schimmelcultures, 1980; 136p.
Sterken HAM, Van den Ende G, Linskens HF, Van Griensven LJLD. De oorzaak van champignonkwekerslong. Isolatie van microorganismen uit de lucht. De Champignoncultuur 1985; 29: 61–65.
Van Loon PCC, Cox AL, Wuisman OPJM, Burgers SLGE, Van Griensven LJLD. Detection of IgG antibodies against Shii-take (Lentinus edodes) spore antigens by enzyme linked immunosorbent assay. J Occup Med 1992; 34: 1097–1101.
Hawkes R, Niday E, Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 1982; 119: 142–47.
Genstat 5 Committee. Genstat 5 reference manual. Oxford: Clarendon Press, 1987.
Rylander R. Lung diseases caused by organic dusts in the farm environment. Am J Ind Med 1986; 10: 221–27.
Küster E. Genus IIThermoactinomyces Tsiklinsky (1899, 501). In: Buchanan RE, Gibbons NE, eds. Bergey's manual of determinative bacteriology. Baltimore: The Williams and Wilkins Co, 1974: 855–56.
Pridham TG, Tresner HD. Genus IStreptomyces Waksman and Henrici (1943, 339). In: Buchanan RE, Gibbons NE, eds. Bergey's manual of determinative bacteriology. Baltimore: The Williams and Wilkins Co, 1974: 748–829.
Küster E. Genus IV.Thermomonospora Henssen (1957, 398). In: Buchanan RE, Gibbons NE, eds. Bergey's manual of determinative bacteriology. Baltimore: The Williams and Wilkins Co, 1974: 858–59.
Gravesen S. Atlas of moulds in Europe causing respiratory allergy. Copenhagen: Forlaget ASK, 1984.
Lacey J, Pepys J, Cross T. Actinomycete and fungus spores as respiratory allergens. In: Shapton DA, Board RG, eds. Safety in microbiology. London: Academic Press, 1972: 151–84.
Kurup VP, Mantyjärvi RA, Terho EO, Ojanen TA, Kalbfleisch JH. Circulating IgG antibodies against fungal and actinomycete antigens in the sera of farmer's lung patients from different countries. Mycopathologia 1987; 98: 91–99.
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Van den Bogart, H.G.G., Van den Ende, G., Van Loon, P.C.C. et al. Mushroom worker's lung: Serologic reactions to thermophilic actinomycetes present in the air of compost tunnels. Mycopathologia 122, 21–28 (1993). https://doi.org/10.1007/BF01103705
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DOI: https://doi.org/10.1007/BF01103705