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Two-dimensional immunoelectrophoresis of histoplasmin and a purified polysaccharide-protein antigen ofHistoplasma capsulatum

  • Human And Animal Mycology
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Abstract

Crude histoplasmin and a polysaccharide-protein complex (PPC-histo) antigens obtained from culture filtrates ofHistoplasma capsulatum were analyzed by single and tandem two-dimensional immunoelectrophoresis (TD-IEP) using a rabbit hyperimmune anti-histoplasmin polyvalent serum. Single TD-IEP showed 14 arc precipitates for histoplasmin. Continuity of arcs 2, 6, and 7, and 9 and 10 was observed, suggesting a different polymeric configuration of the same antigen. This was also confirmed in tandem TD-IEP of histoplasmin with homologous (PPC-histo) and heterologous PPC's fromBlastomyces dermatitidis, Paracoccidioides brasiliensis andCoccidioides immitis. Tandem TD-IEP of histoplasmin and PPC-histo displayed a similar antigenic pattern to histoplasmin alone, being arcs 1 and 3 more evident and apparently present only in histoplasmin and PPC-histo. Tandem TD-IEP showed common antigens among the other heterologous fungal purified antigens, and seems useful to observe the multiplicity of antigens present in fungal preparations and to identify those precipitates (arcs 1 and 3) that are predominant in the purified preparation.

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Authors and Affiliations

  1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510, México DF, México

    C. Toriello, J. A. Jiménez-Montiel, M. R. Reyes-Montes & M. L. Taylor

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  1. C. Toriello
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  2. J. A. Jiménez-Montiel
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  3. M. R. Reyes-Montes
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  4. M. L. Taylor
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Toriello, C., Jiménez-Montiel, J.A., Reyes-Montes, M.R. et al. Two-dimensional immunoelectrophoresis of histoplasmin and a purified polysaccharide-protein antigen ofHistoplasma capsulatum . Mycopathologia 122, 7–13 (1993). https://doi.org/10.1007/BF01103703

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  • DOI: https://doi.org/10.1007/BF01103703

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