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Gastric colonization withCandida albicans

  • Human And Animal Mycology
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Abstract

We adapted a rat model of gastrointestinal candidiasis for studies of in vivo gastric colonization withCandida albicans. Whereas normal rats cleared a single intragastric inoculum of 5×106 C. albicans from the stomach within 4 hours, rats pretreated with chloramphenicol and gentamicin achieved stable gastric colonization for at least 5 days after administration of this inoculum. We next used this model to study host modifications hypothesized to alter gastric colonization. A first group received dilute HCl 4 hr before yeast inoculation, to induce acute superficial gastric erosions; another group was treated with glucocorticosteroid beginning 12 days before yeast inoculation; and another group received famotidine therapy beginning 3 days before yeast inoculation, to neutralize gastric acidity. Recovery of yeasts from stomachs was significantly different from the control group only in rats treated with steroids; greater colonization was found in the rats so treated. In a final group of experiments, we attempted to inhibit in vivo gastric colonization with yeasts by preincubation of yeasts in vitro with a polyclonal antiserum raised in rabbits against heat-killedC. albicans. We were not able to demonstrate inhibition of gastric colonization by preincubation with this antiserum in this model system.

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Authors and Affiliations

  1. Infectious Diseases Section, Department of Medicine, University of Oklahoma Health Sciences Center and the Medical Service, Oklahoma City Department of Veterans Affairs Medical Center, Oklahoma City, USA

    Ronald A. Greenfield MD & Wnedy A. Joyce

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  1. Ronald A. Greenfield MD
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  2. Wnedy A. Joyce
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Greenfield, R.A., Joyce, W.A. Gastric colonization withCandida albicans . Mycopathologia 122, 1–5 (1993). https://doi.org/10.1007/BF01103702

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  • DOI: https://doi.org/10.1007/BF01103702

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