Abstract
Twenty-nineFusarium species isolated from various sources in different districts of Taiwan were tested for their ability to produce fumonisins in corn cultures. OnlyFusarium moniliforme produced fumonisin B1 (FB1) and fumonisin B2 (FB2). The finding that the other 28Fusarium species produced neither FB1 nor FB2 is preliminary because only one strain per species was studied. The detection of FB1 and FB2 in cultures ofF. moniliforme was demonstrated by TLC and HPLC, and FB1 was further confirmed by mass spectrometry. In a separate experiment, in which 38 strains ofF. moniliforme were tested for fumonisins, approximately 66% (25/38) produced FB1 and/or FB2. Of the 25 strains, 14 produced only FB1 and 11 produced both FB1 and FB2, and the amounts of FB1 and FB2 produced by different strains varied greatly. This is the first report that fumonisins are found in corn cultures experimentally infected withF. moniliforme strains from Taiwan. It is safe to assume that fumonisin producing strains ofF. moniliforme are widely distributed among the economic crops such as corn, rice, sugarcane, and sorghum throughout the Island.
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Abbreviations
- FB1 :
-
Fumonisin B1
- FB2 :
-
Fumonisin B2
- OPA:
-
o-phthalidialdehyde
References
Bezuidenhout SC, Gelderblom WCA, Gorst-Allman CP, Horak RM, Marasas WFO, Spiteller G, Vleggaar R. Structure elucidation of the fumonisins mycotoxins fromFusarium moniliforme. J Chem Soc Chem Commun 1988: 743–45
Gelderblom WCA, Jaskiewicz K, Marasas WFO, Thiel PG, Horak RM, Vleggaar R, Kriek NPJ. Fumonisins — novelmycotoxins with cancer promoting activity produced byFusarium moniliforme. Appl Environ Microbiol 1988; 54: 1806–11.
Thiel PG, Shephard GS, Sydenham EW, Marasas WFO, Nelson PE, Wilson TM. Levels of fumonisins B1 and B2 in feeds associated with confirmed cases of equine leukoencephalomalacia. S Agric Food Chem 1991; 39: 109–11.
Voss KA, Norred WP, Plattner RD, Bacon CW. Hepatotoxicity and renal toxicity in rats or corn samples associated with field cases of equine leukoencephalomalacia. Food Chem Toxical 1989; 27: 89–96.
Wilson TM, Ross PF, Rice LG, Osweiler, Nelson HA, Owens DL, Plattner RD, Reggiardo C, Noon TH, Pickrell JW. Fumonisin B1 levels associated with an epizootic equine leukoencephalomalacia. J Vet Diagn Inrest 1990; 2: 217–21.
Ross PF, Nelson PE, Richard JL, Osweiler GD, Rice LG, Plattner RD, Wilson TM, Production of fumonisins byFusarium moniliforme andFusarium proliferatum isolates associated with equine leukoencephalomalacia and a pulmonary edema syndrome in swine. Appl Environ Microbiol 1990; 56: 3225–6.
Sydenham EW, Gelderblom WCA, Thiel PG, Marasas WFO. Evidence for the natural occurrence of fumonisin B1, a mycotoxin produced byFusarium moniliforme in corn. J Agric Food Chem 1990; 38: 285–90.
Nelson PE, Plattner RD, Shackelford DD, Desjardins AE. Production of fumonisins byFusarium moniliforme strains from various substrates and geographic areas. Appl Environ Microbiol 1991; 57: 2410–2.
Nelson PE, Plattner RD, Shackelford DD, Desjardin AE. Fumonisin B1 production byFusarium species other thanF. moniliforme in sectionLiseola and by some related species. Appl Environ Microbiol 1992; 58: 984–9.
Thiel PG, Marasas WFO, Sydenham EW, Shephard GS, Gelderblom WCA, Nieuwenhuis JJ. Survey of fumonisin production byFusarium species. Appl Environ Microbiol 1991; 57: 1089–93.
Tseng TC. Mycotoxins produced byFusarium spp. of Taiwan. Bot Bull Acad Sin 1993; 34: 261–69.
Nask SM, Snyder WC. Quantitative estimations by plate counts of propagules of the bean root rotFusarium in soil. Phytopathology 1962; 52: 567–2.
Nelson PE, Toussoun TA, Marasas WFO.Fusarium Species: An Illustrated manual for identification. University Park, PA: The Pennsylvania State University Press, 1983.
Booth C: GenusFusarium. Kew, Surrey: Commonwealth Mycol Inst, 1971.
Snyder WC, Hansen HN. The species concept inFusarium. Am J Bot 1940; 27: 64–7.
Gerlach W, Nirenberg H. The GenusFusarium — A Pictorial Atlas. Mitt Biol Bundesanst. Land — Forstwirtsch. Berl — Dahlem 1982; 209: 1–406.
Shephard GS, Sydenham EW, Thiel PG, Gelderblom WCA. Quantitative determination of fumonisins B1 and B2 by high-performance liquid chromatography with fluorescence detector. J Liq Chromatogr 1990; 13: 2077–87.
Sydenham EW, Marasas WFO, Shephard GS, Thiel PG, Hirooka EY. Fumonisin concentrations in Brazilian feeds associated with field outbreaks of confirmed and suspected animal mycotoxicoses. J Agri Food Chem 1992; 40: 994–7.
Chelkowski J. Lew H.Fusarium species ofLiseola section — occurrence in cereals and ability to produce fumonisins. Microbiol Alim Nutr 1992; 10: 49–53.
Chen J, Mirocha CJ, Xie W, Hogge L, Olson D. Production of the mycotoxin fumonisin B1 byAlternaria alternata f. sp.lycopersici. Apply Environ Microbiol 1992; 58: 3928–31.
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Tseng, TC., Lee, KL., Deng, TS. et al. Production of fumonisins byFusarium species of Taiwan. Mycopathologia 130, 117–121 (1995). https://doi.org/10.1007/BF01103460
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DOI: https://doi.org/10.1007/BF01103460