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Extracellular proteases ofHendersonula toruloidea Scytalidium hyalinum andScytalidium japonicum

  • Human And Animal Mycology
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Abstract

Proteolytic activity was demonstrated in all the six clinical isolates ofHendersonula toruloidea, five ofScytalidium hyalinum and one ofS. japonicum, screened, by using bovine serum albumin as the substrate. Assay of crude enzyme filtrates showed that enzyme activity increased with the age of the organism reaching a peak after twelve days of growth. The optimum temperature and pH for maximal enzyme activity were 37 °C and 5.6 respectively. The maximum dry weight of mycelia recorded wereH. toruloidea, 135 mg;S. hyalinum, 100 mg; andS. japonicum 103 mg/50 ml broth after 22 days of growth. Proteases may play a role in the pathogensis of infection caused by these fungi by hydrolyzing keratinized tissues such as stratum corneum and nails of humans.

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References

  1. Apodaca G, McKerrow JH. Expression of proteolytic activity by cultures ofTricophyton rubrum. J Med Vet Mycol 1990; 28: 159–71.

    Google Scholar 

  2. Hauck H, Storepova M. Extracellular protease ofTricophyton rubrum and clinical picture of tinea. Mykosen 1987; 30: 25–27.

    Google Scholar 

  3. Minocha V, Pasricha JS, Mohapatra LN, Kandhari KC. Proteolytic activities of dermatophytes and its role in pathogenesis of skin lesions Sabouraudia 1972: 10: 79–85.

  4. Takiuchi T, Higuchi D, Sei Y, Koga M. Isolation of an extracellular proteinase (keratinase) fromMicrosporum canis. Sabouraudia 1982; 20: 281–88.

    Google Scholar 

  5. Meevootison V, Niederpruem DJ. Control of exocellular proteases in dermatophytes and especiallyTrichophyton rubrum. Sabouraudia 1979; 17: 91–106.

    Google Scholar 

  6. Okafor JI, Gugnani HC, Testratke D, Yangcon BG. Extracellular enzyme activities ofBasidiobolus andConidiopolus isolates on solid media. Mykosen 1986; 30: 404–7.

    Google Scholar 

  7. Reuchel R. Candida acid proteinase: Evidence for its contribution to virulence ofCandida species. 11th Congress of International Society for Human and Animal Mycology Montreal, Canada, June 1991, p. 37.

  8. Rhodes JC, Amlung TW. The elastinolytic proteinase ofAspergillus flavus is not glycosylated. J Med Vet Mycol 1991; 29: 407–11.

    Google Scholar 

  9. Kothary MH, Chase Jr J, MacMillian JD. Correlation of elastase production by some strains ofAspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice. Infec Immun 1991; 43: 320–25.

    Google Scholar 

  10. Gugnani HC, Oyeka CA. Foot infections due toHendersonula toruloidea andScytalidium hyalinum in coal miners. J Med Vet Mycol 1989; 27: 169–79.

    Google Scholar 

  11. Moore MK. Skin and nail infections by non-dermatophytic filamentous fungi. Mykosen 1978; 21: 128–32.

    Google Scholar 

  12. Moore MK. Morphological and Physiological studies of isolates ofHendersonula toruloidea Nattrass cultured from human skin and nail samples. J Med Vet Mycol 1988; 26: 25–39.

    Google Scholar 

  13. Allison VY, Hay RT, Campbell CK.Hendersonula toruloidea: Infection in Tabogo. Br J Dermatol. 1984; 111: 371–72.

    Google Scholar 

  14. Hay RJ, Moore MK. Clinical features of superficial fungal infections caused byHendersonula toruloidea andScytalidium hyalinum. Br J Dermatol 1984; 110: 677–83.

    Google Scholar 

  15. Moore MK.Hendersonula toruloidea andScytalidium hyalinum infection in London, U.K. J Med Vet Mycol 1986; 24: 219–30.

    Google Scholar 

  16. Udagawa S, Kiyosh T, Takafumi H.Scytalidium japonicum, a new species, the causal agent of cattle bronchiolitis. Mycotaxon 1986; 25: 279–86.

    Google Scholar 

  17. Renold H, Fasold H, Staib F. Purification and Characterization of proteolytic enzymes fromCandida albicans. Biochem Biophys Acta 1968; 167: 399–406.

    Google Scholar 

  18. Sanyal AK, Das SK, Banerjee AB. Purification and characterization of an exocellular proteinase fromTrichophyton rubrum J Med Vet Mycol 1985; 23: 165–178.

    Google Scholar 

  19. Upton ME, Fogarty WM. Production and purification of thermostable amylase and protease ofThermomonospora viridia. Appl Environ Microbiol 1977; 33: 59–64.

    Google Scholar 

  20. Yu RT, Harmon SR, Blank F. Isolation and purification of an extracellular keratinase ofTrichophyton mentagrophytes. J Bacteriol 1968; 96: 1435–36.

    Google Scholar 

  21. Grappel SF, Blank F. Role of keratinases in dermatophytosis, I: Immune responses in guinea pig immunized with keratinase. Dermatologia 1972; 145: 245–55.

    Google Scholar 

  22. Sutton, BC, Dyko BJ. Revision of Hendersonula. Mycol Res 1989, 93: 466–88.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

    C. A. Oyeka

  2. Department of Microbiology, University of Nigeria, Nsukka, Nigeria

    H. C. Gugnani

Authors
  1. C. A. Oyeka
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  2. H. C. Gugnani
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Oyeka, C.A., Gugnani, H.C. Extracellular proteases ofHendersonula toruloidea Scytalidium hyalinum andScytalidium japonicum . Mycopathologia 130, 67–70 (1995). https://doi.org/10.1007/BF01103451

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  • DOI: https://doi.org/10.1007/BF01103451

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