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The influence of post-filtration washing on the in vitro assay ofCandida albicans adherence to human buccal epithelial cells

  • Human And Animal Mycolocy
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Abstract

A simple in vitro assay technique was used to determine the effect of post-filtration washing on the adherence ofC. albicans (NCPF 3736) to human buccal epithelial cells (BEC). Washing was carried out with a range of volumes of phosphate buffered saline (PBS), viz. 0, 5, 10 and 20 ml, at a standard flow rate. Both the number ofC. albicans adherent to BEC and the percentage of BEC with adherentC. albicans were significantly decreased (p<0.001 for each of these measures) after washing with 5 ml PBS. Further increases in the volume of PBS did not significantly decrease either measure of adherence. These data indicate that only a small volume of PBS, 5 ml, is required to achieve the removal of non-adherentC. albicans from the surface of BEC. The result of the adherence assay is not significantly affected by increasing the volume of PBS used. It is concluded that considerable savings in time may be made through using only a small (5 ml) volume of washing buffer at a standard flow rate.

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Abbreviations

BEC:

buccal epithelial cells

PBS:

phosphate buffered saline

MEM:

Eagle's minimum essential medium

NCPF:

National collection of pathogenic fungi

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Medicine and Surgery, University Dental Hospital of Manchester, Higher Cambridge Street, M15 6FH, Manchester, UK

    Elizabeth D. Theaker

  2. Department of Oral and Maxillofacial Medicine and Surgery Cell and Structural Biology, Turner Dental School, University of Manchester Dental Hospital, Manchester

    D. B. Drucker

  3. Department of Public Health and Epidemiology, University of Manchester, Manchester, UK

    A. C. C. Gibbs

Authors
  1. Elizabeth D. Theaker
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  2. D. B. Drucker
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  3. A. C. C. Gibbs
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Theaker, E.D., Drucker, D.B. & Gibbs, A.C.C. The influence of post-filtration washing on the in vitro assay ofCandida albicans adherence to human buccal epithelial cells. Mycopathologia 121, 11–15 (1993). https://doi.org/10.1007/BF01103348

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  • DOI: https://doi.org/10.1007/BF01103348

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