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Effect of macrophage blockade on the resistance of inbred mice toParacoccidioides brasiliensis infection

  • Human And Animal Mycology
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Abstract

The effect of macrophage blockade on the natural resistance and on the adaptative immune response of susceptible (B10.D2/oSn) and resistant (A/Sn) mice toParacoccidioides brasiliensis infection was investigated. B10.D2/oSn and A/Sn mice previously injected with colloidal carbon were infected ip with yeast cells to determine the 50% lethal dose, and to evaluate the anatomy and histopathology, macrophage activation, antibody production and DTH reactions. Macrophage blockade rendered both resistant and susceptible mice considerably more susceptible to infection, as evidenced by increased mortality and many disseminated lesions.P. brasiliensis infection and/or carbon treatment increased the ability of macrophages from resistant mice to spread up to 25 days after treatment. In susceptible mice the enhanced spreading capacity induced by carbon treatment was impaired at all assayed periods except at 1 week after infection. Macrophage blockade enhanced DTH reactions in resistant mice, but did not alter these reactions in susceptible mice, which remained anergic. To the contrary, macrophage blockade enhanced specific antibody production by susceptible mice, but did not affect the low levels produced by resistant mice. The effect of macrophage blockade confirms the natural tendency of resistant animals to mount DTH reactions in the course of the disease and the preferential antibody response developed by susceptible mice afterP. brasiliensis infection. On the whole, macrophage functions appear to play a fundamental role in the natural and acquired resistance mechanisms toP. brasiliensis infection.

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Abbreviations

CMI:

cellular-mediated immunity

DTH:

delayed-type hypersensitivity

LD50 :

50% lethal dosis

MPS:

mononuclear phagocytic system

PCM:

paracoccidioidomycosis

SRBC:

sheep red blood cells

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Authors and Affiliations

  1. Seção de Imunologia, Instituto Adolfo Lutz, São Paulo, Brasil

    Raquel dos Anjos Fazioli

  2. Departamento de Patologia, Faculdade de Medicina de Botucatu, UNESP, São Paulo, Brasil

    Maura Moscardi-Bacchi & Marcello Franco

  3. Departamento de Immunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, CEP 05508-900, 2415, São Paulo, SP, Brasil

    Suely Sanae Kashino, Raquel dos Anjos Fazioli, Lucia Mary Singer-Vermes, Eva Burger & Vera Lucia Garcia Calich

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  1. Suely Sanae Kashino
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  2. Raquel dos Anjos Fazioli
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  3. Maura Moscardi-Bacchi
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  5. Lucia Mary Singer-Vermes
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  6. Eva Burger
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  7. Vera Lucia Garcia Calich
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Kashino, S.S., Fazioli, R.d.A., Moscardi-Bacchi, M. et al. Effect of macrophage blockade on the resistance of inbred mice toParacoccidioides brasiliensis infection. Mycopathologia 130, 131–140 (1995). https://doi.org/10.1007/BF01103095

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  • DOI: https://doi.org/10.1007/BF01103095

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