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Effect of amphotericin B on the lipids of five different strains ofCryptococcus neoformans

  • Human And Animal Mycology
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Abstract

Cells of five strains ofCryptococcus neoformans were obtained for partial analysis of lipid composition. Quantitative analysis of lipids and sterols were completed, as well as qualitative analysis of sterols by thin-layer chromatography and by the ultraviolet spectra. Such determinations were made on cells cultured in the absence and presence of amphotericin B at sub-MIC (minimal inhibitory concentration) levels. Marked alterations of the lipid and sterol contents were observed in the amphotericin B — treated cells. Moreover, ergosterol disappeared in these antibiotic-exposed cells. It is concluded that amphotericin B altered the lipid profiles, especially sterols ofC. neoformans.

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  1. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Caixa Postal 486, Belo Horizonte, 31.270-901, Minas Gerais, Brazil

    Sarah Piancastelli Franzot & Júnia Soares Hamdan

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  1. Sarah Piancastelli Franzot
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  2. Júnia Soares Hamdan
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Franzot, S.P., Hamdan, J.S. Effect of amphotericin B on the lipids of five different strains ofCryptococcus neoformans . Mycopathologia 128, 85–89 (1994). https://doi.org/10.1007/BF01103014

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  • DOI: https://doi.org/10.1007/BF01103014

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