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Studies with the high voltage electron microscope of normal, degenerating, and Golgi impregnated neuronal processes

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Journal of Neurocytology

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Summary

Synaptic relationships between neuronal processes have been studied in thick sections (up to 1.0 μm) with high voltage electron microscopy. Some of the problems that arise in the study of such thick sections have been considered.

  1. (1)

    Where two parts of a single process are in continuity through a narrow stalk, as a dendritic stem and one of its spines, the continuity can generally not be seen if the stalk is included within the section or is crossed in the section by other processes.

  2. (2)

    Membranes that are viewed face-on cannot be seen in the thick sections and, therefore, processes belonging to two separate nerve cells may appear as though they are in cytoplasmic continuity.

  3. (3)

    Synaptic junctions that are viewed face-on cannot be seen in the thick sections, and junctions ying close to the same angle are also invisible; the relative number of invisible junctions depends upon a number of factors, including the section thickness and the size and contrast properties of the junctional specializations.

Two methods of marking neuronal processes have been explored: (i) degenerating axons that are undergoing the dark degenerative change can be recognized readily within the thick sections. However, they stand out less clearly than in thin sections. (2) Neuronal processes that have been impregnated by an extra-rapid modification of the Golgi method can be easily traced in thick sections. It is possible to study some of the synaptic contacts made by these impregnated processes, especially if the processes have been only lightly impregnated or if the silver dichromate deposits are secondarily removed.

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References

  • Blackstad, T. W. (1965) Mapping of experimental axon degeneration by electron microscopy of Golgi preparations.Zeitschrift für Zellforschung und Mikroskopische Anatomic 67, 819–834.

    Google Scholar 

  • Blackstad, T. W. (1970) Electron microscopy of Golgi preparations for the study of neuronal relations. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. andEbesson, S. O. E.), pp. 186–215. Berlin: Springer.

    Google Scholar 

  • Carasso, N., Delaunay, M. andFavard, P. (1973) Obtention et coloration de coupes épaisses pour la microscopie électronique a haute tension.Journal de Microscopie 16, 257–68.

    Google Scholar 

  • Chan-Palay, V. andPalay, S. L. (1972) High voltage electron microscopy of rapid Golgi preparations. Neurons and their processes in the cerebellar cortex of monkey and rat.Zeitschrift für Anatomie und Entwicklungsgeschichte 137, 125–52.

    Google Scholar 

  • Chan-Palay, V. andPalay, S. L. (1972) The form of velate astrocytes in the cerebellar cortex of monkey and rat: High voltage electron microscopy of rapid Golgi preparations.Zeitschrift für Anatomie und Entwicklungsgeschichte 138, 1–19.

    Google Scholar 

  • Christensen, B. N. (1973) Procion Brown: An intracellular dye for light and electron microscopy.Science 182, 1255–6.

    PubMed  Google Scholar 

  • Clark, A. W. andBranton, D. (1968) Fracture faces in frozen outer segments from the guinea pig retina.Zeitschrift für Zellforschung und Mikroskopische Anatomie 91, 586–603.

    Google Scholar 

  • Colonnier, M. (1968) Synaptic patterns on different cell types in the different laminae of the cat visual cortex. An electron microscope study.Brain Research 9, 268–87.

    PubMed  Google Scholar 

  • Dennison, M. E. (1971) Electron stereoscopy as a means of classifying synaptic vesicles.Journal of Cell Science 8, 525–539.

    Google Scholar 

  • Favard, P., Ovtracht, L. andCarasso, N. (1971) Observation de specimens biologiques en microscopie électronique á haute tension. I. Coupes epaisses.Journal de Microscopie 12, 301–316.

    Google Scholar 

  • Favard, P. andCarasso, N. (1973) The preparation and observation of thick biological sections in the high voltage electron microscope.Journal of Microscopy 97, 59–81.

    PubMed  Google Scholar 

  • Frasca, J. M. andParks, V. R. (1965) A routine technique for double staining ultrathin sections using uranyl and lead salts.Journal of Cell Biology 25, 157–61.

    PubMed  Google Scholar 

  • Gray, E. G. (1959) Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study.Journal of Anatomy (London) 93, 420–33.

    Google Scholar 

  • Gray, E. G. (1963) Electron microscopy of presynaptic organelles of the spinal cord.Journal of Anatomy (London) 97, 101–6.

    Google Scholar 

  • Gray, E. G. andGuillery, R. W. (1966) Synaptic morphology in the normal and degenerating nervous system.International Review of Cytology 19, 111–182.

    PubMed  Google Scholar 

  • Gray, E. G. andWillis, R. A. (1968) Problems of electron stereoscopy of biological tissue.Journal of Cell Science 3, 309–26.

    Google Scholar 

  • Guillery, R. W. (1967) A light and electron microscopical study of neurofibrils and neurofilaments at neuro-neuronal junctions in the dorsal lateral geniculate nucleus of the cat.American Journal of Anatomy 120, 583–604.

    Google Scholar 

  • Guillery, R. W. (1969) The organization of synaptic interconnections in the laminae of the dorsal lateral geniculate nucleus of the cat.Zeitschrift für Zellforschung und Mikroskopische Anatomie 96, 1–38.

    Google Scholar 

  • Guillery, R. W. (1970) Light and electron microscopical studies of normal and degenerating axons. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. andEbbesson, S. O. E.), pp. 77–105. Berlin: Springer.

    Google Scholar 

  • Guillery, R. W. (1971) Patterns of synaptic interconnections in the dorsal lateral geniculate nucleus of cat and monkey: a brief review.Vision Research, Supplement 3, 211–27.

    Google Scholar 

  • Guillery, R. W. andScott, G. L. (1971) Observations on synaptic patterns in the dorsal lateral geniculate nucleus of the cat: The C laminae and the perikaryal synapses.Experimental Brain Research 12, 184–203.

    Google Scholar 

  • Hama, K. andPorter, K. R. (1969) An application of high voltage electron microscopy to the study of biological materials.Journal de Microscopie 8, 149–58.

    Google Scholar 

  • Hudson, B. andMakin, M. J. (1970) The optimum tilt angle for electron stereo-microscopy.Journal of Physics, E. Scientific Instruments 3, 311.

    Google Scholar 

  • Le Vay, S. (1973) Synaptic patterns in the visual cortex of the cat and monkey. Electron microscopy of Golgi preparations.Journal of Comparative Neurology 150, 53–86.

    PubMed  Google Scholar 

  • Locke, M. andKrishnan, N. (1971) Hot alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections.Journal of Cell Biology 50, 550–7.

    PubMed  Google Scholar 

  • Nankizell, J. F. (1963) The theory of electron stereo-microscopy.Optik. Zeitschrift für das Gesamte Gebiet der Licht- und Elektronenoptik 20, 172–98.

    Google Scholar 

  • Peters, A., Hinds, P. L. andVaughn, J. E. (1971) Extent of stain penetration for electron microscopy.Journal of Ultrastructure Research 36, 37–45.

    PubMed  Google Scholar 

  • Peters, A. andPalay, S. L. (1966) The morphology of laminae A and A1 of the dorsal nucleus of the lateral geniculate body of the cat.Journal of Anatomy (London) 100, 451–86.

    Google Scholar 

  • Ramón-Moliner, E. andFerrari, J. (1972) Electron microscopy of previously identified cells and processes within the central nervous system.Journal of Neurocytology 1, 85–100.

    PubMed  Google Scholar 

  • Ris, H. (1969) Use of the high voltage electron microscope for the study of thick biological specimens.Journal de Microscopie 8, 761–6.

    Google Scholar 

  • Shelton, P. J., Horridge, G. A. andMeinertzhagen, I. A. (1971) Reconstruction of synaptic geometry and neural connections from serial thick sections examined by the medium high voltage electron microscope.Brain Research 29, 373–7.

    PubMed  Google Scholar 

  • Stell, W. K. (1964) Correlated light and electron microscope observations on Golgi preparations of goldfish retina.Journal of Cell Biology 23, 89A.

    Google Scholar 

  • Stretton, A. O. W. andKravitz, E. A. (1969) Neuronal geometry: determination with a technique of intracellular dye injection.Science 162, 132–4.

    Google Scholar 

  • Szentágothai, J. (1964) The use of degeneration methods in the investigation of short neuronal connections.Progress in Brain Research 14, 1–32.

    Google Scholar 

  • Uchizono, K. (1965) Characteristics of excitatory and inhibitory synapses in the central nervous system of the cat.Nature (London) 207, 642–3.

    Google Scholar 

  • Venable, J. H. andCoggeshall, R. (1965) A simplified lead citrate stain for use in electron microscopy.Journal of Cell Biology 25, 407–8.

    PubMed  Google Scholar 

  • Yamada, E. andIshikawa, H. (1972) High voltage electron microscopy for thick sections of biological materials combined with molecular tracers.Proceedings of the 30th Annual Meeting of Electron Microscope Societies of America, p. 480.

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Authors and Affiliations

  1. Department of Anatomy, University of Wisconsin, 53706, Madison, Wisconsin

    G. L. Scott & R. W. Guillery

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  1. G. L. Scott
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  2. R. W. Guillery
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Scott, G.L., Guillery, R.W. Studies with the high voltage electron microscope of normal, degenerating, and Golgi impregnated neuronal processes. J Neurocytol 3, 567–590 (1974). https://doi.org/10.1007/BF01097625

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  • DOI: https://doi.org/10.1007/BF01097625

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