Summary
Various organs of three species of monkey were screened histochemically for esteroproteases usingN-acethyl-l-methionine-α-naphthylester (α N-O-met) as the substrate and also for enzymes with kallikrein-like activity usingd-Val-Leu-Arg-4-methoxy-2-naphthylamide as the substrate. Characteristic differences were found in the localization of the reaction products obtained with both substrates. In the main salivary glands, esteroproteases (α N-O-met reactivity) were found in mucous cells (submandibular gland), intercalated duct cells (parotid gland), acinar cells (sublingual gland), striated and interlobular duct cells (all glands). They were also localized in superficial lining epithelial cells of the digestive system, in liver cells, and acinar cells of the pancreas.
Enzymes with kallikrein-like activity were found only in the striated and interlobular duct cells of salivary glands, in acinar cells of the pancreas, and in proximal tubular cells of the kidney. Free cells (including mast cells) normally distributed in the connective tissue of various organs showed reactivity towards α N-O-met. Some of these cells were also reactive against Val-Leu-Arg-4-MNA.
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Al-Gailani, M., Garrett, J. R., Kidd, A., Kyriacou, K. &Leite, P. (1980a) Localization of esteroproteases in resting salivary glands from different species and the effects of the organophosphorus inhibitor E 600.Histochemistry 66, 59–74.
Al-Gailani, M., Garrett, J. R., Kidd, A., Kyriacou, K. &Leite, P. (1980b) Histochemical demonstration of esteroproteases in salivary glands.J. Physiol. 303, 34–5.
Arnold, W. H., Ørstavik, T. B. &Holck, M. (1983a) A4-methoxy-2-naphthylamide substrate for the histochemical localization of esteroproteases in the submandibular gland of the rat.Histochem. J. 15, 139–46.
Arnold, W. H. (1983b) A comparative study on the localization of kallikrein and kallikrein-like activity in different organs. InKininogenases Kallikrein VI (edited byHaberland G. L., Rohen, J. W., Fritz, H. andHuber, P.), pp. 59–73. Stuttgart: Schattauer Verlag.
Billing A. G., Moriwaki, C., Peret, M. W. &Schachter, M. (1982) Studies on the localization of some serine proteases (kallikreins, acrosin) in the digestive and reproductive tract.J. Physiol. 328, 49.
Brandzaeg, P., Gautvik, H. M., Nustad, K. &Pierce, I. V. (1976) Rat submandibular gland kallikreins: purification and cellular localization.Br. J. Pharmac. 56, 155–67.
Cohen, S. (1960) Purification of a nerve growth factor promoting protein from the mouse salivary gland and its neuro-cytotoxic antiserum.Proc. Natn. Acad. Sci. USA 46, 302–11.
Dietl, T. &Breiner, M. (1983) Active enzyme staining of tissue kallikrein and related protease in submandibular glands and mast cells. InKininogenases, Kallikrein VI (edited byHaberland, G. L., Rohen, J. W., Fritz, H. andHuber, P.) Stuttgart Schattauer Verlag.
Fritz, H., Kruck, J., Russe, I. &Liebich, H. G. (1979) Immunofluorescence studies indicate that the basic trypsin-kallikrein inhibitor of bovine organs (Trasylol) originates from mast cells.Hoppe-Seyler's Z. physiol. Chem. 360, 437–44.
Garrett, J. R., Smith, R. E., Kidd, A., Kyriacou, K. &Gabske, R. I. (1982) Kallikrein-like activity in salivary glands using a new tripeptide substrate, including preliminary secretory studies and observations on mast cells.Histochem. J. 14, 967–80.
Greene, L. A., Shooter, E. M. &Varon, S. (1969) Subunit interaction and enzymatic activity of mouse 7S nerve growth factor.Biochemistry 8, 3735–41.
Holt, S. J. (1959) Factors governing the validity of staining methods for enzymes, and their bearing upon the Gomori acid phosphatase technique.Expl Cell Res. 7, 1–27.
Hopsu-Havu, V. K., Riekkinen, P. J. &Ekfors, Z. O. (1967) Studies on the alkaline trypsin-like enzymes in rat submandibular gland and saliva.Acta odont. scand. 25, 657–67.
Ledoux, St., Gutowska, J., Garcia, R., Thibault, G., Cantin, M. &Genest, J. (1983) Immunohistochemical localization of tonin rat salivary glands and kidney.Histochemistry 76, 329–39.
Lexow, U., Großarth, C. &Von Deimling, O. (1979) Histochemical demonstration of mouse submandibular stereoproteases with new chromogenic substrate.Histochemistry 60, 327–34.
Mason, A. J., Evans, B. A., Cox, D. R., Shine, J. &Richards, R. J. (1983) Structure of mouse kallikrein gene family suggests a role in specific processing of biologically active peptides.Nature 303, 300–7.
Mobley, W. C., Schenker, A. &Shooter, E. M. (1976) Characterization and isolation of proteolytically modified nerve growth factor.Biochemistry 15, 5543–51.
Nustad, K., Gautvik, K. M. &Ørstavik, T. B. (1979) Radioimmunoassay of rat submandibular gland kallikrein and the detection of immunoreactive antigen in blood. InKinins II, Vol. 120,A Biochemistry, Pathophysiology and Clinical Aspects (edited byFuji, S. Moriya, H. andSuzuki, T.), pp. 225–34. New York: Plenum.
Otto, J. &Von Deimling, O. (1980) Classification of multiple forms of submandibular gland esteroproteases of the house mouse. (M. musclulus).Comp. Biochem. Physiol. 65, 615–22.
Ørstavik, T. B., Brandzaeg, P., Nustad, K. &Halvorsen, K. M. (1975) Cellular localization of kallikrein in the rat submandibular and sublingual salivary glands. Immunofluorescent tracing related to histological characteristics.Acta histochem. 54, 183–92.
Ørstavik, T. B., Nustad, K., Brandzaeg, P. &Pierce, I. V. (1976) Cellular origin of urinary kallikreins.J. Histochem. Cytochem. 24, 1037–39.
Ørstavik, T. B. &Glenner, G. G. (1978) Localization of kallikrein and its relation to other trypsin-like esterases in the rat pancreas. A comparison with the submandibular gland.Acta physiol. scand. 103, 384–93.
Ørstavik, T. B., Carretero, O. A., Mayashi, H. &Johansen, L. (1982) Immunohistochemical localization of tonin and its relation to kallikrein in rat salivary glands.J. Histochem. Cytochem. 30, 1123–29.
Porcelli, G., Cozzari, C., Diiorio, M., Croxatto, R. H. &Angeletti, P. (1976) Isolation and partial characterization of kallikrein from submaxillary glands.Ital. J. Biochem. 25, 337–48.
Schachter, M., Peret, M. W., Moriwaki, C. &Rodrigues, J. A. (1980) Localization of kallikrein in submandibular gland of cat, guinea pig, dog and man by the immunoperoxidase method.J. Histochem. Cytochem. 28, 1295–300.
Schaller, E. &Von Deimling, O. (1979) Methionine-α-naphthyl ester, a useful chromogenic substrate for esteroproteases of the mouse submandibular gland.Annls Biochem. 93, 251–6.
Schenkein, J., Boesman, M., Tokarsky, E., Fishman L. &Levy, M. (1969) Proteases from mouse submaxillary gland.Biochem. biophys. Res. Commun. 36, 156–65.
Simson, J. A. V., Spicer, S. S., Chao, J., Grimm, L. &Margolius, H. S. (1989) Kallikrein-localization in rodent salivary glands and kidney with the immunoglobulinenzyme bridge technique.J. Histochem. Cytochem. 27, 1567–76.
Taylor, J. M., Cohen, S. &Mitchell, W. M. (1970) Epidermal growth factor: high and low molecular weight forms.Proc. Natn. Acad. Sci. 67, 164–71.
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Arnold, W.H. Comparative studies on the localization of esteroproteases and kallikrein-like activity in primate organs. Histochem J 16, 755–769 (1984). https://doi.org/10.1007/BF01095280
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DOI: https://doi.org/10.1007/BF01095280