Summary
Freeze-dried frozen sections prepared from unfixed rat lateral prostate were examined by X-ray microanalysis in an attempt to establish thein vivo distribution of endogenous ions. Poor morphological resolution was a limiting factor in the analysis of subcellular regions of the tissue. Glutaraldehyde fixation prior to cryo-sectioning resulted in considerable loss of elements. The results are discussed and compared with those obtained from ultrathin sections of tissue treated with potassium pyroantimonate. Using the latter method, it was possible to demonstrate a subcellular distribution pattern for the element zinc and to correlate the metal with specific organelles. It is considered that, unlike a number of other tissues, the rat prostate does not lend itself readily to cryoultramicrotomy as a preparative regime.
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Timms, B.G., Chandler, J.A. Endogenous elements in the prostate. An X-ray microanalytical study of freeze-dried frozen sections and histochemical localization of zinc by potassium pyroantimonate. Histochem J 16, 733–754 (1984). https://doi.org/10.1007/BF01095279
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DOI: https://doi.org/10.1007/BF01095279