Plant Foods for Human Nutrition

, Volume 30, Issue 3–4, pp 259–270 | Cite as

Ox-Dapro: theLathyrus sativus neurotoxin

  • B. K. Misra
  • S. P. Singh
  • G. K. Barat


The present knowledge of the neurotoxin inLathyrus sativus is summarized, the involvement of the toxin in causing neurolathyrism in humans discussed, and future research needs suggested.


Plant Physiology Present Knowledge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Adiga PR, Rao SLN, Sarma PS (1963) Some structural features and neurotoxic action of a compound fromL. sativus seeds. Curr Sci 32:153Google Scholar
  2. 2.
    Anderson LAP, Albert H, Simonsen JL (1924) Studies on lathyrism. Indian J Med Res 12:613–643Google Scholar
  3. 3.
    Barrow MV, Simpson CF, Miller J (1974) Lathyrism. Q Rev Biol 49:101–128Google Scholar
  4. 4.
    Bell EA, O'Donovan JP (1966) The isolation of α and Y-oxalyl derivatives ofl-α, γ-diaminobutyric acid from seeds ofLathyrus latifolius and the detection of the α-oxalyl isomer of the neurotoxin alpha amino-beta-oxalyl aminopropionic acid which occurs together with the neurotoxin in this and other species. Phytochemistry 5:1211–1219Google Scholar
  5. 5.
    Bhagwat K (1946) Toxic effects in guinea-pigs of diets containing large proportions ofLathyrus sativus. Indian J Med Res 34:299–304Google Scholar
  6. 6.
    Cheema PS, Malathi K, Padmanaban G, Sarma PS (1968) Neurotoxicity of beta-N-oxalyll-alpha-beta-diaminopropionic acid, theLathyrus sativus neurotoxin. Proc Nutr Soc (Inida) (Sept)Google Scholar
  7. 7.
    Cheema PS, Malathi K, Padmanaban G, Sarma PS (1969) Neurotoxicity of β-N-oxalyll-α, β-diaminopropionic acid, the neurotoxin from the pulseLathyrus sativus. Biochem J 112:29–33Google Scholar
  8. 8.
    Cheema PS, Malathi K, Padmanaban G (1969) Neurotoxic action of β-N-oxalyl-l-α, β-diaminopropionic acid in acidotic adult rats. Indian J Biochem 6:146Google Scholar
  9. 9.
    Cheema PS, Padmanaban G, Sarma PS (1970) Biochemical characterization of β-N-oxalyll, α, β-diaminopropionic acid, theLathyrus sativus neurotoxin as an excitant amino acid. J Neurochem 17:1295–1298Google Scholar
  10. 10.
    Cheema PS, Padmanaban G, Sarma PS (1971) Mechanism of action of β-N-oxalyll-α, β-diaminopropionic acid, theL. sativus neurotoxin. J Neurochem 18:2137–2144Google Scholar
  11. 11.
    Dahiya BS (1976) Seed morphology as an indicator of low neurotoxin inLathyrus sativus L. Qual Plant Plant Foods Hum Nutr 25:391Google Scholar
  12. 12.
    Das AD, Sahani K, Medda AK (1974) Incidence of lathyrism in man. Indian J Physiol Allied Sci 28:100–106Google Scholar
  13. 13.
    Ganapathy KT, Dwivedi MP (1961) Studies on clinical epidemiology of lathyrism. Indian Counc Med Res Rep, New DelhiGoogle Scholar
  14. 14.
    Haskell BE, Bowlus SB (1976) A new synthesis of 1-2-amino-3 oxalyl aminopropionic acid, theL. sativus neurotoxin. J Org Chem 41:159–160Google Scholar
  15. 15.
    Indian Council of Medical Research (ICMR) New Delhi (1964) Lathyrism a preventable paralysis. Hyderabad, India: National Research LaboratoryGoogle Scholar
  16. 16.
    Jakob E, Patel AJ, Ramchandra CV (1967) Effect of neurotoxin fromL. sativus on glutamic acid metabolism in chick brain. J Neurochem 14:1091Google Scholar
  17. 17.
    Jeswani LM, Lal BM, Shiv Prakash (1970) Studies on the development of low neurotoxin (β-N-oxalyl amino alanine) lines inLathyrus sativus (Khesari). Curr Sci 39:518Google Scholar
  18. 18.
    Johnston GAR (1974) Neurotoxic amino acids. In: Simpson LL (ed) Neuropoisons: their pathological action, vol 2. New York: DR Curtis, pp 179–205Google Scholar
  19. 19.
    Johnston GAR, Llyod HJ (1967) Oxalyl coenzyme A synthetase and the neurooxin β-N-oxalyll-α, β-diaminopropionate. Aust J Biol Sci 20:1241–1244Google Scholar
  20. 20.
    Malathi K, Padmanaban G, Rao SLN, Sarma PS (1967) Studies on the biosynthesis of β-N-oxalyl-l-α, β-diaminopropionic acid, theLathyrus sativus neurotoxin. Biochim Biophys Acta 141:71–78.Google Scholar
  21. 21.
    Malathi K, Padmanaban G, Sarma PS (1970) Biosynthesis of β-N-oxalyl-l-α, β-diaminopropionic acid, theLathyrus sativus neurotoxin. Phytochemistry 9:1603–1610Google Scholar
  22. 22.
    Misra BK (1978) Biochemical investigations on the neurotoxin, β-N-oxalyll, α, β-diaminopropionic acid. PhD thesis, Meerut University, MeerutGoogle Scholar
  23. 23.
    Misra BK, Pande PC, Barat CK (1979) Negative correlation between crude protein and β-N-oxalyl,l-α, β-diaminopropionic acid (ODAP) inLathyrus sativus germplasm. Plant Biochem J 6:124–127Google Scholar
  24. 24.
    Mohan VS, Nagarajan V and Gopalan C (1966) simple practical procedure for the removal of toxic factors inL. sativus. Indian J Med Res 54:410–414Google Scholar
  25. 25.
    Murti VVS, Seshadri TR (1964) Toxic amino acids of plants. Curr Sci 33:323–329Google Scholar
  26. 26.
    Murti VVS, Seshadri TR, Venkitasubramanian TA (1964) Neurotoxic compounds of the seeds ofLathyrus sativus. Phytochemistry 3:73–78Google Scholar
  27. 27.
    Nagarajan V, Gopalan C (1968) Variation in the neurotoxin β-(N) oxalylamino alanine content inLathyrus sativus samples from Madhya Pradesh. Indian J Med Res 56:95–99Google Scholar
  28. 28.
    Nagarajan V, Mohan VS, Gopalan C (1965) Toxic factors inLathyrus sativus. Indian J Med Res 53:269Google Scholar
  29. 29.
    Nagarajan V, Mohan VS (1967) A simple and specific method for the detection of adulteration withLathyrus sativus. Indian J Med Res 55:1011–1014Google Scholar
  30. 30.
    Nagarajan V, Mohan VS, Gopalan C (1967–68) Annual report. Hyderabad: Nutrition Research Laboratory, p 24Google Scholar
  31. 31.
    National Institute of Nutrition, Hyderabad (1976) Annual reportGoogle Scholar
  32. 32.
    Olney JW, Misra CH, Vesa R (1976) Brain and retinal damage fromLathyrus excitotoxin, β-N-oxalyl-l-α, β-diaminopropionic acid. Nature 264:659Google Scholar
  33. 33.
    Panda NC, Mitra A, Kedary S (1972) Experimental lathyrism in chicks. Indian J Anim Sci 42:949–951Google Scholar
  34. 34.
    Parker AJ, Mehta T, Zarghami NS, Cusick PK, Haskell BE (1979) Acute neurotoxicity of theLathyrus sativus neurotoxin,l-3-oxalylamino-2-amino-propionic acid in the squirrel monkey. Toxicol Appl Pharmacol 47:135–144Google Scholar
  35. 35.
    Passios CS, Demopoulos T (1962) Human lathyrism, a clinical and skeletal study. Clin Orthopol 23:236Google Scholar
  36. 36.
    Patwardhan VN (1952) Nutrition in India. Bombay: Indian J Med SciGoogle Scholar
  37. 37.
    Przybylska J, Rymowlej T (1965) Free amino acids in different organs of sixteenLathyrus species. Genet Pol 6: 91–124Google Scholar
  38. 38.
    Querishi MY, Pilbeam DJ, Evans CS, Bell EA (1977) The neurolathyrogen, α-amino-β-oxalylaminopropionic acid in legume seeds. Phytochemistry 16:477–479Google Scholar
  39. 39.
    Rao SLN, Sarma PS (1967) Neurotoxic action of β-N-oxalyl-l, α, β-diaminopropionic acid. Biochem Pharmacol 16:218–220Google Scholar
  40. 40.
    Rao SLN, Sarma PS, Mani KS, Rao R, Srinivasachari S (1967) Experimental neurolathyrism in monkeys. Nature (London) 214:610–611Google Scholar
  41. 41.
    Rao SLN, Adiga PR, Sarma PS (1964) Isolation and characterisation of β-N-oxalyll-α, β-diaminopropionic acid, a neurotoxin from the seeds ofLathyrus sativus. Biochemistry 3:432–436Google Scholar
  42. 42.
    Rao SLN, Malathi K, Sarma PS (1969) Lathyrism. World Rev Nutr Diet 10:214–238Google Scholar
  43. 43.
    Rao DR, Hariharan K, Vijayalakshmi KR (1974) Specific enzymic procedure for the determination of neurotoxic components (derivatives ofl-α, β-diaminopropionic acid) inLathyrus sativus. J Food Chem 22:1146–1148Google Scholar
  44. 44.
    Roy DN (1969) Biosynthesis of β-(N)-oxalyl amino alanine: evidence that serine is not the precursor. Indian J Biochem 6:147–149Google Scholar
  45. 45.
    Roy DN, Rao BSN (1968) Distribution of the alpha and beta isomers in some Indian varieties ofLathyrus. Curr Sci 37:395–396Google Scholar
  46. 46.
    Roy DN, Nagarajan V, Gopalan C (1963) Production of neurolathyrism in chicks by the injection ofLathyrus sativus concentrates. Curr Sci 32:116–118Google Scholar
  47. 47.
    Sarma PS, Padmanaban G (1969) In: Liener IE (ed) Toxic constituents of plant food stuffs. New York: Academic Press, p 267Google Scholar
  48. 48.
    Shiv Prakash, Misra BK, Adsule RN, Barat GK (1977) Distribution of β-N-oxalyll-α, β-diaminopropionic acid in different tissues of agingLathyrus sativus plant. Biochem Physiol Pflanz 171:369–374Google Scholar
  49. 49.
    Siddiq EA, Majid R, Nerkar YS (1978) Studies on the cytotoxic effects of lathyrogen. New Bot 2:76–80Google Scholar
  50. 50.
    Singh HD, Shiv Prakash, Misra BK, Lal BM (1968) Investigations on neurotoxin substance causing lathyrism inLathyrus sativus. In: Second annual workshop conference on pulse crops. New Delhi: Indian Agricultural Research Institute, pp 1–5Google Scholar
  51. 51.
    Somayajulu PLN, Barat GK, Shiv Prakash, Misra BK, Srivastava YC (1975) Breeding of low toxin containing varieties ofLathyrus sativus. Proc Nutr Soc (India) 19:35–39Google Scholar
  52. 52.
    Stockman R (1929) Lathyrism. J Pharmacol 37:43–53Google Scholar
  53. 53.
    Striepler M, Cohn DF, Hirano A, Schujaman E (1978) The central nervous system in a case of neurolathyrism. Neurology 27:1176–1178Google Scholar
  54. 54.
    Strong FM (1966) Naturally occurring toxic factors in plants and animals used as food. Can Med Assoc J 194:568–573Google Scholar
  55. 55.
    Swaminathan MS (1969) Role of mutation breeding in a changing agriculture IAEA SM-121/61. In: Induced mutations in plants. Vienna: IAEA, pp 719–734Google Scholar
  56. 56.
    Watkins JC, Curtis DR, Biscoe TJ (1966) Central effects of β-N-oxalyl-l, α, β-diaminopropionic acid and otherLathyrus factors. Nature 211:637Google Scholar

Copyright information

© Martinus Nijhoff/Dr. W. Junk Publishers 1981

Authors and Affiliations

  • B. K. Misra
    • 1
  • S. P. Singh
    • 1
  • G. K. Barat
    • 1
  1. 1.Biochemistry DivisionIndian Agricultural Research InstituteNew DelhiIndia

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