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Plant Foods for Human Nutrition

, Volume 30, Issue 3–4, pp 223–233 | Cite as

Growth, blood chemistry and histology of rats fed zincsupplemented rapeseed protein concentrates

  • B. G. Shah
  • E. A. Nera
  • P. C. Verdier
  • J. L. Beare-Rogers
  • J. D. Jones
  • K. Anjou
  • R. Ohlson
Article

Abstract

Two rapeseed protein concentrates (RPC) supplemented with zinc oxide were fed to male and female rats at two levels providing approximately half or all of the protein in a purified basal diet containing 20% protein (N×6.25). Control groups were given either a purified basal diet with casein or a laboratory chow diet. After 16 weeks, there were no significant (P<0.05) differences in body weight except the male rats fed the casein control diet had higher body weights than did other male rats. No significant differences were observed in organ weights including thyroids, hematology, bone marrow differential counts, serum glucose, urea nitrogen, total protein, albumin/globulin ratio, cholesterol, transaminases or alkaline phosphatase. The levels of serum vitamins A and E of females fed some of the RPC diets were less than in those fed the caseincontrol diet, but equal to those in females fed laboratory chow diet. Females fed either RPC or casein in the semi-purified diets, which contained zinc oxide, exhibited kidney calcification.

Keywords

Alkaline Phosphatase Control Diet Serum Glucose Basal Diet Serum Vitamin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Agren G, Eklund A (1972) Rapeseed protein fractions III. The nutritive value of a detoxified protein concentrate prepared from rapeseed (Brassica napus L.) by hydraulic processing. J Sci Food Agric 23:1457–1462Google Scholar
  2. 2.
    American Institute of Nutrition (1977) Report of the Ad Hoc Committee on standards for nutritional studies. J Nutr 107:1340–1348Google Scholar
  3. 3.
    Anjou K, Krook C, Fecske A, Ohlson R (1976) Production of rapeseed protein concentrate for human consumption. Canadian Patent 999186Google Scholar
  4. 4.
    Anjou K (1979) Antinutritional components in rapeseed and their effect on pregnant rats. In: Proceedings of the 5th International Rapeseed Conference, Malmö, Sweden, vol 2. Organizing Committee of the 5th International Rapeseed Conference, Malmö, pp 133–137Google Scholar
  5. 5.
    Beare-Rogers JL, Nera EA, Graig BM (1972) Cardiac lipids in rats and gerbils fed oils containing C22 fatty acids. Lipids 7:548–552Google Scholar
  6. 6.
    Bernhart FW, Tomarelli RM (1966) A salt mixture supplying the National Research Council estimates of the mineral requirements of the rat. J Nutr 89:495–500Google Scholar
  7. 7.
    Department of National Health and Welfare (1973) Nutrition Canada, national survey report. Ottawa, Ontario: Information Canada, p 29Google Scholar
  8. 8.
    Eklund A, Agren G (1978) Effect of dietary rapeseed protein concentrate on the contents of α-tocopherol and zinc in serum, liver and tibia of rats. Nutr Metab 22:218–230Google Scholar
  9. 9.
    Freund JE, Livermore PE, Miller I (1960) Manual of experimental statistics. Englewood Cliffs NJ: Prentice-Hall, pp 16–18Google Scholar
  10. 10.
    Lewis AJ, Aherne FX, Hardin RT (1978) Reproductive performance of sows fed low glucosinolate (Tower) rapeseed meal. Can J Anim Sci 58:203–208Google Scholar
  11. 11.
    Loew FM, Doige CE, Manns JG, Searcy GP, Bell JM, Jones JD (1976) Evaluation of dietary rapeseed protein concentrate flours in rats and dogs. Toxicol Appl Pharmacol 35:257–267Google Scholar
  12. 12.
    National Research Council (1972) Nutrient requirements of laboratory animals, Publ no. 10. Washington DC: National Academy of Sciences, pp 56–93Google Scholar
  13. 13.
    McKinnon PJ, Bowland JP (1977) Comparison of low glucosinolate-low erucic acid rapeseed meal (C. V. Tower), commercial rapeseed meal and soybean meal as sources of protein for starting, growing and finishing pigs and young rats. Can J Anim Sci 57:663–678Google Scholar
  14. 14.
    McLaughlan JM, Jones JD, Shah BG, Beare-Rogers JL (1975) Reproduction in rats fed protein concentrate from mustard or rapeseed. Nutr Rep Int 11:327–335Google Scholar
  15. 15.
    Peters JM (1967) Effects of absolute starvation and refeeding on organ weights and water contents of albino rats. Growth 31:191–203Google Scholar
  16. 16.
    Peters JM, Krijnen CJ (1966) Organ weights and water contents of rats fed purified diets. Growth 30:99–107Google Scholar
  17. 17.
    Protein-Calorie Advisory Group (PAG) of the United Nations System (1972) PAG guideline no. 6. Preclinical testing. Revised 13 March 1972. New York: United NationsGoogle Scholar
  18. 18.
    Shah BG, Giroux A, Belonje B, Jones JD (1979) Beneficial effect of zinc supplementation on reproduction in rats fed rapeseed protein concentrate. Nutr Metab 23:275–285Google Scholar
  19. 19.
    Shah BG, Giroux A, Belonje B, Jones JD (1980) Evaluation of rapeseed protein concentrate as a source of protein in a zinc supplemented diet for young rats. J Agric Food Chem 28:36–39Google Scholar
  20. 20.
    Timm NH (1975) Multivariate analysis. Monterey CA: Brooks/ColeGoogle Scholar
  21. 21.
    Wetter LR, Youngs CG (1976) A thioureau-UV assay for total glucosinolate content in rapeseed meals. J Am Oil Chem Soc 53:162–164Google Scholar

Copyright information

© Martinus Nijhoff/Dr. W. Junk Publishers 1981

Authors and Affiliations

  • B. G. Shah
    • 3
  • E. A. Nera
    • 3
  • P. C. Verdier
    • 3
  • J. L. Beare-Rogers
    • 3
  • J. D. Jones
    • 1
  • K. Anjou
    • 2
  • R. Ohlson
    • 2
  1. 1.Food Research InstituteAgriculture CanadaOttawaCanada
  2. 2.AB Karlshamns OljehrabrikerKarlshamnsSweden
  3. 3.Food DirectorateHealth Protection BranchOttawaCanada

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