Abstract
Qualitative and quantitative analysis of polar lipids in sun flower (Helianthus annuus L.) seed is reported in the present study. Ten classes of polar lipids i.e. phosphatidic acid, phosphatidyl inositol, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl glycerol, diphosphatidyl glycerol, digalactosyl diglyceride, monogalactosyl diglyceride, esterified sterol glycoside and free fatty acids have been identified. Phosphatidyl choline and phosphatidyl inositol formed the major phosphatide fractions. Only four fatty acids i.e. palmitic, stearic, oleic and linoleic acids were present in measurable amounts in the total polar lipids. There was a great variation in the fatty acid composition in different classes of polar lipids. Arachidic acid was present in all the individual phosphatide fractions. Phosphatidyl glycerol was highly unsaturated followed by esterified sterolglycoside, phosphatidic acid, phosphatidyl ethanolamine + phosphatidyl glycerol, diphosphatidyl glycerol and monogalactosyl diglyceride.
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Baddiley, J., Buchanan, J. G., Handschumacher, R. E. & Prescott, J. F. (1956). Chemical studies on biosynthesis of purine nucleotides,J. Chem. Soc. 3:2818.
Bligh, E. G. & Dyer, W. G. (1959). A rapid method of total lipid extraction and purification,Can. J. Biochem. Physiol. 37:911–17.
Bregoff, H. M., Roberts, E. & Delwiche, C. C. (1953). Paper chromatography of quaternary ammonium bases and related compounds.J. Biol. Chem. 205:265–74.
Fewster, M. E., Burns, B. J. & Mead, J. F. (1969). Quantitative densitometric thin layer chromatography of lipids using copper acetate reagent.J. Chromatog. 43:120–26.
Gardner, H. W. (1968). Preparative isolation of mono galactosyl and digalactosyl diglycerides by thin layer chromatography.J. Lipid Res. 9:139–41.
Hopkins, C. Y. & Chisholm, M. J. (1961). Development of oil in the seed of Helianthus annuus, L.Can. J. Biochem. Physiol. 39:1481–87.
Jacin, H. & Mishkin, A. R. (1965). Separation of carbohydrate on borate impregnated silica gel G plates.J. Chromatog. 18:170–73.
Luddy, F. E., Barford, R. A., Herb, S. F. & Paul, M. (1968). A rapid quantitative procedure for the preparation of methyl esters of butterfat and other fats.J. Am. Oil Chem. Soc. 45:549.
Mangold, H. K. (1961). Thin layer chromatography of lipidsJ. Am. Oil. Chem. Soc. 38:708–27.
Martensson, E. (1966). Neutral glycolipids of human kidney. Isolation, identification & fatty acid composition.Biochem. Biophys. Acta. 116:296–308.
Nichols, B. W. (1964). ‘New biochemical separations’ by James, A. T. & Morris, L. J., D. Van Nostrand Co. Ltd. London, P. 321.
Pruthi, T. D. & Bhatia, I. S. (1970). Lipids in cereals (Pennisetum typhoideum)J. Sci. Fd Agric. 21:419–22.
Sims, R. P. A. & Larose, J. A. G. (1962). The use of iodine vapours as a general detecting agent in the thin layer chromatography of lipids.J. Am. Oil Chem. Soc. 39:232.
Vaskovsky, V. E. & Kostetsky, E. Y. (1968). Modified spray for the detection of phospholipids on thin layer chromatography.J. lipid Res. 9:396.
Vidal, A. A. (1956). Physical and chemical characteristics of oils at different stages of maturity of the seeds of several plant species.Rev. fac. agron. La Plata 32:159. Quoted fromChem. Abstr. (1956) 25:264a.
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Grewal, S.S., Sukhija, P.S. & Bhatia, I.S. Polar lipids of sun flower (Helianthus annuus L.) seeds. Plant Food Hum Nutr 28, 211–218 (1978). https://doi.org/10.1007/BF01093950
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DOI: https://doi.org/10.1007/BF01093950