Abstract
The effect of light intensity on the synthesis of oxalate and cation concentration in leaves, stems and roots ofC. amarànthicolor L. was investigated. It appears that oxalic acid is synthesized in leaves from metabolites of both photosynthetic and non-photosynthetic origin. In roots the effect of light on the oxalic acid synthesis was different to that of leaves and stems. Cation uptake seems to increase with increase in oxalic acid production.
The influence of applications of superphosphate (single) urea, calcium ammonium nitrate, potassium chloride and salinity on the oxalate production and cation make up ofC. album L. was also studied. All the nutrients except at low levels of calcium ammonium nitrate depressed slightly the oxalate synthesis in leaves. The influence of the applications of sodium chloride and potassium chloride was more pronounced although the higher levels of both of these salts gave almost an equal yield of oxalic acid. These results suggest that the soil nutrients can only partly regulate the oxalic acid production in this plant. The findings also support the belief that chloride or the other anions, if available, are absorbed, compete for cations and depress the oxalate synthesis.
Zusammenfassung
Untersucht wurde der Einfluβ der Lichtintensität auf die Oxalatsynthese und die Kationenkonzentration in Blättern, Stengeln und Wurzeln von Chenopodium amaranthicolor L. Es scheint, daβ in den Blättern die Oxalsäure aus Metaboliten synthetisiert wird, die sowohl photosynthetischen als auch nicht photosynthetischen Ursprungs sein können. Der Einfluβ des Lichtes auf die Oxalsäuresynthese in den Wurzeln weicht von dem in den Blättern und Stengeln ab. Die Kationenaufnahme scheint mit der Zunahme der Oxalsäureproduktion anzústeigen.
Des weiteren wurde der Einfluβ von Superphosphat, Harnstoff, Kalkammon-Salpeter, Kaliumchlorid und Natriumchlorid auf die Oxalatbildung und die Kationenaufnahme bei Chenopodium album untersucht. Alle hier angeführten Nährstoffe, mit Ausnahme der geringsten Gabe Kalkammon-Salpeter, wirkte depressiv auf die Oxalatsynthese in den Blättern. Natrium- und Kaliumchlorid hatten den stärksten Einfluβ, wobei zwischen diesen beiden Salzen kein Unterschied bestand. Diese Ergebnisse legen den Gedanken nahe, daβ die Bodennährstoffe nur teilweise die Oxalsäureproduktion in dieser Pflanze regulieren. Auβerdem stützen sie die Annahme, daβ Chloride oder andere Anionen, sofern sie verfügbar sind, absorbiert werden und als Konkurrenten um die Kationen auftreten und sich somit auf die Oxalatsynthese hemmend auswirken.
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References
Anderson, W., Hollins, J.G. & Bond, P.S. (1971). The composition of tea infusions examined in relation to the association between mortality and water hardness.J. Hyg. 69:1–15.
Baker, C.J.L. (1952). Determination of oxalates in fresh plant materialAnalyst. 77:340–344.
Becker, M. (1964). Dependence of the formation of oxalic acid in spinach (Spinacea oleracea) upon the form of the nitrogen available.Arch. Gartenbau 12:539–551.
Chang, C. & Beevers, H. (1968). Biogenesis of oxalates in plant tissuesPlant Physiol. 43:1821–1828.
Derderian, M.D. (1961). Determination of calcium and magnesium in plant material with EDTA.Anal. Chem. 33:1796.
Devuyst, A., Arnould, R., Vanstaen, L., Vanbelle, M., Berrack, W. & Moreeles, A. (1963). Influence of fertilization with calcium nitrate on the oxalic acid content of sugar beet leaves.Agricultura 10:635–642.
Ehrendorfer, K. (1964). Influence of minerals, especially phosphorus, on the content of oxalic acid in spinach.Phosphorsäure. 24:189–199.
Gericke, S. (1965). Contents of phosphoric and oxalic acids in beet leaves.Phosphorsäure 25:263–280.
Gherand Sanhita, Ganga Vishnu Sri Krishnadas, Bombay (1957) p. 64.
Gilbert, S.C., Shear, C.B. & Group, C.M. (1951). The effects of the form of nitrogen and the amount of base supply on the organic acids of tung plant.Plant. Physiol. 26:750–756.
James, L.F. (1968). Serum electrolyte, acid base balance and enzyme changes in acute Halogeton glomeratus poisoning in sheep.Can. J. Comp. Med. 32:539–543.
James, M.P., Seawright, A.A. & Steele, D.P. (1971). Experimental acute ammonium oxalate poisoning in sheep.Aust. Vet. J. 47:9–17.
Jeghers, H. & Murphy, R. (1945). Practical aspect of oxalate metabolism.New. Eng. J. Med. 233:208–215.
Joy, K.W. (1954). Accumulation of oxalate in tissues of sugar beet and effects of nitrogen supply.Ann. Bot. 28:689–701.
Kitchen, J.W., Burns, E.E. & Langeston, R. (1964). The effect of light, temperature and ionic balance on oxalate formation in spinach.Proc. Am. Soc. Hort. 85:465–470.
Millered, A, Mortan, R.K. & Wells, R.W.E. (1962). Role of isocitrate lyase in the synthesis of oxalic acid in plants.Nature 196:955–956.
Millered, A., Mortan, R.K. & Wells, R.W.E. (1963). Oxalic acid synthesis in the shoots ofOxalis-pescaprae. The precursors of glycolic and glyoxylic acid.Biochem. J. 88:276–281.
Oke, O.L. (1970). Toxic chemicals in Nigerian food stuffs.Indian J. Nutr. Dietet. 7:119–129.
Osmond, C.B. (1963). Oxalates and ionic equilibria in Australian salt bushes (Atriplex).Nature 198:503–504.
Osmond, C.B. (1967). Acid metabolism in Atriplex I. Regulation of oxalate synthesis by the apparent excess cation absorption in leaf tissue.Aust. J. Biol. Sci. 20:575–587.
Osmond, C.B. & Avadhani, P.N. (1968). Acid metabolism in Atriplex II. Oxalate synthesis during acid metabolism in dark.Aust. J. Biol. Sci. 21:917–927.
Pirie, N.W. (1969). Complementary ways of meeting the worlds protein need.Proc. Nutr. Soc. 28:255–263.
Richardson, K.E. & Tolbert, N.E. (1961). Oxidation of glyoxylic acid to oxalic acid by glycolic acid oxidase.J. Biol. Chem. 236:1280–1284.
Singh, P.P., Kothari, L.K., Sharma, D.C. & Saxena, S.N. (1972). An assessment of the nutritional value of foods in relation to their oxalic acid content.Am. J. Clin. Nutr. 25:1147–1152.
Singh, P.P. & Saxena, S.N. (1972). Effect of maturity on the oxalate and cation contents of six leafy vegetables.Indian J. Nutr. Dietet. 9:269–276.
Singh, P.P. & Sharma, D.C. (1968). Significance of some leaf flours in animal nutrition. I. Supplementary value of cauliflower and patharchatta leaf flours to the wheat and rice diets.Indian Med. Gaz. 7(9):20–24.
Singh, P.P. & Sharma, D.C. (1968). Significance of some leaf flours in animal nutrition. II. The effect of feeding the diets containing kulfa leaf flour.Indian Med. Gaz. 8(II):28–33.
Singh, P.P., Sharma, D.C. & Sur, B.K. (1969). Value of green leaves as sources of available calcium.Indian J. Med. Res. 57:204–209.
Singh, P.P. & Sur, B.K. (1962). Unknown bound oxalate in the leaves of Bathua (Chenopodium album L.)Curr. Sci. 31:333–334.
Srivastava, S.K. & Krishnan, P.S. (1969). Oxalic acid in higher plants.J. Sci. Indian. Res. 18:220–224.
Tolbert, N.E. (1971). Microbodies- peroxisomes and glyosixomes.Ann. Rev. Pl. Physiol. 22:45–74.
Zarembski, P.M. & Hodgkinson, H.A. (1962). The oxalic acid content of English diets.Brit. J. Nutr. 16:627–634.
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Professor of biochemistry
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Singh, P.P. Influence of light intensity, fertilizers and salinity on oxalate and mineral concentration of two vegetables (Chenopodium album L. and Chenopodium amaranthicolor L.). Plant Food Hum Nutr 24, 115–125 (1974). https://doi.org/10.1007/BF01092728
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DOI: https://doi.org/10.1007/BF01092728