Skip to main content

Variation of the amino acid scores and of the nitrogen-to-protein conversion factors in barley grain as a function of nitrogen content as compared with wheat and rye

Abstract

Barley grains (9 samples from 7 cultivars) with nitrogen contents (N) ranging from 1.45 to 4.01% of dry matter were analysed for their amino acid (AA) composition with high accuracy from six different hydrolysates per sample. AA levels in grain increased as linear functions ofN with correlation coefficients close to unity. A comparison with literature data confirmed that the AA composition of any grain sample of normal barley can be predicted from itsN for all phenotypes and genotypes. AAs in grain protein changed as hyperbolic functions ofN which increased for Phe, Pro and Glx but more or less strongly decreased for the other AAs. By plotting AA scores againstN, barley proteins were shown to be always richer than wheat and rye in Val and Phe + Tyr; sometimes richer than both other species forN<2 (Lys); 2.2 (Leu and Ile); 3.4 (Thr); sometimes intermediate to wheat and rye above the latterN values. They were also intermediate in sulphur AAs forN<1.9 and drastically poorer forN>1.9. However, they were richer than both other species in Trp forN>1.6. The hyperbolic variations of non-protein nitrogen and nitrogen-to-protein conversion factors were determined as a function ofN and also compared with those of wheat and rye.

This is a preview of subscription content, access via your institution.

References

  1. Andersen AJ, Køie B (1975)N fertilization and yield response of high lysine and normal barley. Agronomy J 67: 695–698

    Google Scholar 

  2. Baudet J, Huet JC, Mossé J (1986) Variability and relationships among amino acids and nitrogen in maize grains. J Agric Food Chem 34: 365–370

    Google Scholar 

  3. Baudet J, Huet JC, Mossé J (1987) Interdépendance entre la composition en acides aminés du grain de seigle et son taux d'azote. Agronomie: 7 (in press)

  4. Baudet J, Leclercq P, Mossé J (1971) Sur la richesse en lysine des graines de Tournesol. CR Acad Sci Paris 273D: 1112–1115

    Google Scholar 

  5. Briggs DE (1978) Barley. Chapman and Hall (eds), London

  6. Chery J (1979) Influence de la fertilisation azotée sur le rendement et la qualité du grain de différentes variétés d'orge. In: Seed Protein Improvement of Cereals and Grain Legumes. Proc Symp Neuherberg IAEA. Vienne: IAEA, Vol. 1, pp 283–296

    Google Scholar 

  7. Chung OK, Pomeranz Y (1985) Amino acids in cereal proteins and protein fractions. In: Finley JW, Hopkins DT (eds) Digestibility and Amino Acid Availability in Cereals and Oilseeds. St Paul (Minn) USA: AAC Inc Publ, pp 65–107

    Google Scholar 

  8. Chung OK, Pomeranz Y (1985) Proteins in developing and germinating cereal grains and grain fractions. In: Finley JW, Hopkins DT (eds) Digestibility and Amino Availability in Cereals and Oilseeds, St Paul (Minn) USA: AAC Inc Publ, pp 109–168

    Google Scholar 

  9. Coīc Y, Fauconneau G, Pion R, Busson F, Lesaint C, Labonne F (1963) Influence de l'alimentation minérale sur la composition des protides des graines de céréales (Blé et Orge). Ann Physiol Vég 5: 281–292

    Google Scholar 

  10. Eggum BO (1970) Uber die Abhängigkeit der Proteinqualität von Stickstoffgehalt der Gerste. Z. Tierphysiol Tierernähr Futtermittelk 26: 65–71

    Google Scholar 

  11. Eppendorfer WH (1968) The effect of nitrogen and sulfur on changes in nitrogen fractions of barley plants at various early stages of growth and on yield and amino acid composition of grain. Plant Soil 29: 424–438

    Google Scholar 

  12. Eppendorfer WH (1971) Effects of S, N and P on amino acid composition of field beans (Vicia faba) and responses of the biological value of the seed protein to S-amino acid content. J Sci Food Agric 22: 501–505

    PubMed  Google Scholar 

  13. Eppendorfer WH (1975) Effects of fertilizers on quality and nutritional value of grain protein. In: Fertilizer Use and Protein Production, Proceeding 11th Coll Int Potash Institute, Rönne-Bornholm, Denmark, IPI, Berne

    Google Scholar 

  14. Eppendorfer WH, Bille SW (1974) Amino acid composition as a function of total-N in pea seeds grown on two soils with P and K additions. Plant Soil 41: 33–39

    Google Scholar 

  15. FAO (1973) Energy and protein requirements Food and Agricultural Organization Nurition Meetings Report Series, no 52. Roma: FAO

    Google Scholar 

  16. Finley JW (1985) Reducing variability in amino acid analysis. In: Finley JW, Hopkins DT (eds) Digestibility and amino acid availability in cereals and oilseeds, St Paul (Minn) USA: AAC Inc Publ, pp 15–30

    Google Scholar 

  17. Jones AS, Cadenhead A, Livingstone RM (1968) Variation in the composition of barley and its effect on the performance of pigs. J Sci Food Agric 19: 446–448

    Google Scholar 

  18. Laberge DE, MacGregor AW, Metcalfe DR (1976) Screening for high-lysine cultivars in a barley breeding program. Can J Plant Sci 56: 817–821

    Google Scholar 

  19. Martens H, Bach Knudsen KE (1980) Fractioning barley proteins by computer factor analysis. Cereal Chem 57: 97–105

    Google Scholar 

  20. McGeown P, Maguire MF (1967) Relationships between crude protein content and amino acid composition of Irish barleys. Irish J Agric Res 6: 221–227

    Google Scholar 

  21. Mossé J, Baudet J (1969) Etude intervariétale de la qualité protéique des orges: taux d'azote, composition en acides aminés et richesse en lysine. Ann Physiol Vég 11: 51–66

    Google Scholar 

  22. Mossé J, Baudet J (1977) Relations entre la composition en acides aminés basiques et la teneur en azote de grains d'orge. In: Europ Brewery Conv, Proceed 16th Congress. Elsevier Amsterdam, pp 27–34

    Google Scholar 

  23. Mossé J, Baudet J (1983) Crude protein content and amino acid composition of seeds: variability and correlations. (Qual Plant) Plant Foods Hum Nutr 32: 225–245

    Google Scholar 

  24. Mossé J, Huet JC, Baudet J (1985) The amino acid content of wheat grain as a function of nitrogen content. J Cereal Sci 3: 115–130

    Google Scholar 

  25. Mossé J, Huet JC, Baudet J (1987) Relationships between nitrogen, amino acids and storage proteins inLupinus albus seeds. Phytochemistry 26: 2453–2458

    Google Scholar 

  26. Mossé J, Huet JC, Baudet J (1987) Changements de la composition en acides aminés des grains de pois en fonction de leur taux d'azote. Sci Aliments 7: 301–324

    Google Scholar 

  27. Mossé J, Huet JC, Baudet J (1988) The amino acid composition of Triticale grain as a function of nitrogen content. J Cereal Sci 6: (in press)

  28. Munck L (1981) Barley for food, feed and industry. In: Pomeranz Y, Munck L (eds) Cereals: a renewable resource, theory and practice. Minnesota: AACC, pp 427–459

    Google Scholar 

  29. Pion R (1971) Composition en acides aminés des aliments. Ind Alim Anim 6: 29–36

    Google Scholar 

  30. Pomeranz Y, Robbins GS (1976) Amino acid composition of isogenic lines in barley. J Agric Food Chem 24: 196–198

    PubMed  Google Scholar 

  31. Pomeranz Y, Robbins GS, Gilbertson JT, Booth GD (1977) Effects of nitrogen fertilization on lysine, threonine, and methionine of hulled and hull-less barley cultivars. Cereal Chem 54: 1034–1042

    Google Scholar 

  32. Pomeranz Y, Robbins GS, Smith RT, Craddock JC, Gilbertson JT, Moseman JG (1976) Protein content and amino acid composition of barleys from the World Collection. Cereal Chem 53: 497–504

    Google Scholar 

  33. Rhodes AP, Mathers JC (1974) Varietal differences in the amino acid composition of barley grain during development and under varying nitrogen supply. J Sci Food Agric 25: 963–972

    PubMed  Google Scholar 

  34. Sosulski FW, Wright AT, Hoover R (1986) Evaluation of protein nutritive value in barley and wheat cultivars usingAspergillus flavus. (Qual Plant) Plant Foods Hum Nutr 36: 63–73

    Google Scholar 

  35. Thomke S (1970) Uber die Veränderung des Aminosaüregehaltes der Gerste mit steigendem Sticktoffgehalt. Z Tierphysiol Tierernähr Futtermittelk 27: 23–31

    Google Scholar 

  36. Tkachuk R (1969) Nitrogen-to-protein conversion factors for cereals and oilseed meals. Cereal Chem 46: 419–423

    Google Scholar 

  37. Winkler U, Schön WJ (1980) Amino acid composition of the kernel proteins in barley resulting from nitrogen fertilization at different stages of development. Z Acker-Pflanzenbau 149: 503–512

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Huet, JC., Baudet, J., Bettaieb, L. et al. Variation of the amino acid scores and of the nitrogen-to-protein conversion factors in barley grain as a function of nitrogen content as compared with wheat and rye. Plant Food Hum Nutr 38, 175–188 (1988). https://doi.org/10.1007/BF01091722

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01091722

Key words

  • barley
  • Triticeae
  • grain
  • amino acid composition
  • nitrogen
  • chemical score