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Plant Foods for Human Nutrition

, Volume 33, Issue 2–3, pp 169–172 | Cite as

Determination of polyphenolic compounds in leaf protein concentrates of lucerne and their effect on the nutritional value

  • J. C. Rambourg
  • B. Monties
Article

Abstract

Leaf protein concentrates were prepared by heat coagulation of lucerne ‘brown juice’. A fraction of the phenolics was completely extractable by organic solvents. Coumestrol (which occurs in lucerne leaf proteins) was estimated after progressive elution with solvents and fluorimetry in solution. Significant reductions of both coumestrol content and total flavonoîdes were observed after washing of the concentrates.

Key words

leaf protein concentrate coumestrol polyphenolic compounds lucerne fluorimetry nutritional value 

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References

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    Bickoff EM, Booth AN, Lyman RL, Livingston AL, Thompson CR, Deeds F (1957) Coumestrol, a new oestrogen isolated from forage crops. Science, NY 126:969–70Google Scholar
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    Monties B, Rambourg JC (1978) Présence de flavonoîdes Sensu stricto (Flavones et coumestanes) dans les préparations de protéines extraites de luzerne (Medicago sativa var. Europe). Ann Technol Agric 27 (3):629–654Google Scholar
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    Knuckles BE, de Fremery D, Kohler GO (1976) Coumestrol content of fractions obtained during wet processing of alfalfa. J Agric Food Chem 24 (6):1177–1180Google Scholar
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    Ramboug JC, Monties B (1980) Dosage fluorimétrique et densitométrique du coumestrol. Application au contrôle de la qualité des préparations protéiques de feuilles de luzerne (Medicago sativa). Ann Technol Agric 29 (3):463–475Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1983

Authors and Affiliations

  • J. C. Rambourg
    • 1
  • B. Monties
    • 2
  1. 1.France Luzerne, Complexe AgricoleChalons sur MarneFrance
  2. 2.Laboratoire de chimie biologique et de Photophysiologie I.N.R.A., Institute National AgronomiqueCentre de GrignonThiverval GrignonFrance

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