Summary
Candida wickerhamii produces one endocellular and one exocellular β-glucosidase. Both enzymes are repressed by glucose in the wild-type strain. In the M7 mutant ofC. wickerhamii, which was previously demonstrated to be derepressed for endocellular β-glucosidase biosynthesis, the exocellular β-glucosidase is derepressed and hyperproduced when cellodextrins are added to the culture medium. This enzyme, which was produced constitutively in the wildtype, has thus become inducible in the M7 mutant strain. The interest of this strain for industrial production of β-glucosidases is discussed.
Résumé
Candida wickerhamii produit deux β-glucosidases, l'une endo- et l'autre exocellulaire. Les deux enzymes de la souche sauvage sont réprimées par le glucose. Le mutant M7, chez qui il a été antérieurement constaté que la synthèse de la β-glucosidase endocellulaire est déréprimée, l'enzyme exocellulaire est elle aussi déréprimée et hyper-produite lorsque des cellodextrines sont ajoutées au milieu de culture. Cette enzyme, qui est constitutive chez la souche sauvage, est donc devenue inductible chez le mutant M7. Cette souche est intéressante pour la production industrielle de β-glucosidases.
Resumen
Candida wikerhamii produce dos β-glucosidasas: una endocelular y otra exocelular. Ambos enzimas son reprimidos por glucosa en la cepa salvaje. Al añadir celodextrina al medio de cultivo del mutante M7 deC. wickerhamii, en el cual se ha demostrado ya la desrepresión de la síntesis de β-glucosidasa endocelular, se desreprime la β-glucosidasa extracelular obteniéndose una hiperproducción de este enzima. Dicho enzima que era producido de forma constitutiva en el tipo salvaje, se ha convertido en inducible en la cepa mutante M7. Se discute el interés de esta cepa para la producción industrial de β-glucosidasas.
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References
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Leclerc, M., Gondé, P., Arnaud, A., Ratomahenina, R., Galzy, P. &Nicolas, M. 1984 The enzyme systems in a strain ofCandida wickerhamii Meyer and Yarrow participating in the hydrolysis of cellodextrins.Journal of General and Applied Microbiology 30, 509–521.
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Leclerc, M., Chemardin, P., Arnaud, A. et al. Exocellular β-glucosidase inducibility in a mutant strain ofCandida wickerhamii derepressed for endocellular β-glucosidase production. Mircen Journal 3, 87–90 (1987). https://doi.org/10.1007/BF01090498
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DOI: https://doi.org/10.1007/BF01090498