Plant Systematics and Evolution

, Volume 219, Issue 1–2, pp 127–135 | Cite as

A monophyletic origin of the B chromosomes ofBrachycome dichromosomatica (Asteraceae)

  • Andreas Houben
  • Nicole Thompson
  • Richard Ahne
  • Carolyn R. Leach
  • Dawn Verlin
  • Jeremy N. Timmis


The A and B chromosomes of different karyotype variants (cytodemes A1, A2, A3 and A4) ofBrachycome dichromosomatica were analysed by computer-aided chromosome image analysis and fluorescencein situ hybridisation (FISH). Ribosomal DNA and the B chromosome-specific sequence Bd49 were detected on all B chromosomes. In addition to minor size variation of the Bs, polymorphism of the rDNA and Bd49 position and copy number revealed two major types of B chromosomes. The B chromosomes of all the cytodemes were indistinguishable from each other in length, but that of A3 showed evidence of rearrangements consistent with its long-term geographic isolation. The results presented suggest a monophyletic origin of the B chromosomes ofB. dichromosomatica.

Key words

Asteraceae Brachycome B chromosome polymorphism evolution karyotyping centromere 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adachi J., Watanabe K., Fukui K., Ohmido N., Kosuge K. (1997) Chromosomal location and reorganisation of the 45S and 5S rDNA in theBrachycome lineariloba complex (Asteraceae). J. Plant Res. 110: 371–377.Google Scholar
  2. Ahne R., Huhn P., Laumer N., Zerneke F. (1989) Interaktive rechnergestützte Chromosomenanalyse. Ein Grundsoftwarepaket. Arch. Züchtungsforschung 19: 133–140.Google Scholar
  3. Cabrero J., Lopez-Leon M. D., Gomez R., Castro A. J., Martin-Alganza A., Camacho J. P. M. (1997) Geographical distribution of B chromosomes in the grasshopperEyprypocnemis plorans, along a river basin, is mainly shaped by non-selective historical events. Chromosome Res. 5: 194–198.Google Scholar
  4. Carter C. R., Smith-White S. (1972) The cytology ofBrachycome lineariloba 3. Accessory chromosomes. Chromosoma 39: 361–379.Google Scholar
  5. Carter C. R. (1978) Taxonomy of theBrachycome lineariloba complex (Asteraceae). Telopea 1: 387–393.Google Scholar
  6. Choo K. H. A. (1997) Chromatin Dynamics 97 Centromere DNA dynamics: Latent centromeres and neocentromere formation. Am. J. Hum. Genet 61: 1225–1233.Google Scholar
  7. Cullis C. A. (1979) Quantitative variation of ribosomal RNA genes in flax genotrophs. Heredity 42; 237–246.Google Scholar
  8. Donald T. M., Leach C. R., Timmis J. N. (1995) Ribosomal RNA genes and the B chromosome ofBrachycome dichromosomatica. Heredity 74: 556–561.Google Scholar
  9. Donald T. M., Houben A., Leach C. R., Timmis J. N. (1997) Ribosomal RNA genes specific to the B chromosome inBrachycome dichromosomatica are not transcribed in leaf tissue. Genome 40: 674–681.Google Scholar
  10. Franks T. K., Houben A., Leach C. R., Timmis S. J. N. (1996) The molecular organisation of a B chromosome tandem repeat sequence fromBrachycome dichromosomatica. Chromosoma 105: 223–230.Google Scholar
  11. Gottlob-McHugh S. G., Levesque M., Mackenzie K., Olson M., Yarosh O., Johnson D. A. (1990) Organization of the 5S rDNA genes in the soybean (Glycine max (L.)) Merrill and conservation of the 5S rDNA repeat structure in higher plant. Genome 33: 486–494.Google Scholar
  12. Holmes D. S., Bougourd S. M. (1991) b Chromosome selection inAllium schoenoprasum. II. Experimental populations. Heredity 67: 117–122.Google Scholar
  13. Houben A., Leach C. R., Verlin D., Rofe R., Timmis J. N. (1997a) A repetitive DNA sequence common to the different B chromosomes of the genusBrachycome. Chromosoma 106: 513–519.Google Scholar
  14. Houben A., Belyaev N. D., Leach C. R., Timmis J. N. (1997b) Differences of histone H4 acetylation and replication timing between A and B chromosomes ofBrachycome dichromosomatica. Chromosome Res 5: 223–237.Google Scholar
  15. Ijdo J. W., Wells R. A., Baldini A., Reeders S. T. (1991) Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Research 19: 4780.Google Scholar
  16. John U. P., Leach C. R., Timmis J. N. (1991) A sequence specific to B chromosomes ofBrachycome dichromosomatica. Genome 34: 739–744.Google Scholar
  17. Jones R. N., Rees H. (1982) B chromosomes, 1st edn. Academic Press, London New York.Google Scholar
  18. Jones R. N. (1995) Tansley Review No. 85, B chromosomes in plants. New Phytol. 131: 411–434.Google Scholar
  19. Leach C. R., Donald T. M., Franks T. K., Spiniello S. S. Hanrahan C. F., Timmis J. N. (1995) Organisation and origin of a B chromosome centromeric sequence fromBrachycome dichromosomatica. Chromosoma 103: 708–714.Google Scholar
  20. Lopez-Leon M. D., Cabrero J., Pardo M. C., Viseras E., Camacho J. P. M., Santos J. L. (1993) Generating high variability of B chromosomes in the grasshopperEyprepocnemis plorans. Heredity 71: 352–362.Google Scholar
  21. Martinez P., Vinas A., Bouza C., Castro J., Sanchez L. (1993) Quantitative analysis of the variability of nucleolar organizer regions inSalmo trutta. Genome 36: 1119–1123.Google Scholar
  22. Niwa K., Sakamoto S. (1995) Origin of B chromosomes in cultivated rye. Genome 38: 307–312.Google Scholar
  23. Watanabe K., Carter C. R., Smith-White S. (1975) the Cytology ofBrachycome lineariloba 5. Chromosome relationships and phylogeny of the race A cytodemes (n=2). Chromosoma 52: 383–397.Google Scholar
  24. Watanabe K., Denda T., Suzuki Y., Kosuge K., Ito M., Short P. S., Yahara T. (1994) Chromosomal and molecular evolution in the genusBrachycome (Astereae). In: Hind D. J. N., Beebtje H. J. (eds.) Compositae: Systematics. Proceedings of the international Compositae Conference. Kew, Vol. 1, pp. 705–722.Google Scholar
  25. Wienand U., Feix G. (1980) Zein-specific restriction enzyme fragments of maize DNA. FEBS Lett. 116: 14–16.Google Scholar
  26. Yakura K., Tanifuji S. (1983) Molecular cloning and restriction analysis ofEcoR1-Fragments ofVicia faba rDNA. Plant Cell Physio. 24: 1327–1330.Google Scholar

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Andreas Houben
    • 1
  • Nicole Thompson
    • 2
  • Richard Ahne
    • 3
  • Carolyn R. Leach
    • 1
  • Dawn Verlin
    • 1
  • Jeremy N. Timmis
    • 1
  1. 1.Department of GeneticsThe University of AdelaideAustralia
  2. 2.Department of Crop ProtectionThe University of AdelaideGlen OsmondAustralia
  3. 3.Bundesanstalt für Züchtungsforschung an KulturpflanzenQuedlinburgGermany

Personalised recommendations