Several genotypes, number given within parenthesis, of chickpea, pigeonpea, urd bean, mung bean and soybean, differing in seed characteristics were analyzed for phytic acid, in vitro protein digestibility (IVPD), protein, total phosphorus, and seed size. Phytic acid contents and IVPD values differed significantly among and within these species. Phytic acid content (mg/g) was the highest in soybean (36.4) followed by urd bean (13.7), pigeonpea (12.7), mung bean (12.0) chickpea (9.6). On an average, phytic acid constituted 78.2 percent of the total phosphorus content and this percentage figure was the highest in soybean and the lowest in mung bean. In vitro protein digestibility (IVPD) of pigeonpea and chickpea genotypes varied from 60.4 to 74.4 percent and 65.3 to 79.4 percent, respectively. The IVPD values of genotypes of mung bean, urd bean and soybean ranged from 67.2 to 72.2 percent, 55.7 to 63.3 percent and 62.7 to 71.6 percent, respectively. There was a significant negative correlation between phytic acid and IVPD of these genotypes. Phytic acid was significantly and positively correlated with protein but the magnitude of correlation was very low in chickpea and pigeonpea. Results indicate that the genotypes of pulses with low phytic acid content could be identified and used in breeding program to improve their nutritive value and utilization.
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Deshpande US, Deshpande SS (1991) Legumes. In: Salunkhe DK, Deshpande SS (eds), Foods of plant origin: Production, technology, and human nutrition. New York: Van Nostrand Reinhold, pp 137–300.
Salunkhe DK (1982) Legumes in human nutrition: Current status and future research needs. Curr Sci 51: 387–394.
Singh U, Singh B (1992) Tropical grain legumes as important human foods. Econ Bot 46: 310–321.
Singh U (1991) The role of pigeonpea in human nutrition. In: Uses of tropical grain legumes. Proceedings of a Consultants' Meeting, 27–30 March 1989. Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics.
Liener IE (1976) Legume toxins in relation to protein digestibility: A review. J Food Sci 41: 1076–1081.
Lolas GM, Markakis P (1975) Phytic acid and other phosphorus compounds in beans (Phaseolus vulgaris L.). J Agric Food Chem 23: 13–15.
Deshpande SS, Sathe SK, Salunkhe DK, Cornforth DK (1982) Effects of dehulling on phytic acid, polyphenols and enzyme inhibitors of dry beans (Phaseolus vulgaris L.). J Food Sci 47: 1846–1847.
O'Dell BL, De Boland A (1976) Complexation of phytate with proteins and cations in corn and oilseed meals. J Agric Food Chem 24: 804–808.
Singh U, Singh B, Smith OD (1991) Effect of varieties and processing methods on phytic acid and protein digestibilty of groundnut (Arachis hypogaea) J Food Sci Tech 28: 345–347.
Reddy NR, Sathe SK, Salunkhe DK (1982) Phytates in legumes and cereals. Adv Food Res 28: 1–90.
Singh U, Jambunathan R (1981) Methods for the estimation of protein in pigeonpea (Cajanus cajan L.) and the relationship between whole grain and dhal protein contents. J Sci Food Agric 32: 705–710.
Wheeler EL, Ferrel PE (1971) A method for phytic acid determination in wheat and wheat fractions. Cereal Chem 48: 312–320.
Technicon Industrial Systems (1972) Industrial Method No. 218-72A.
Singh U, Jambunathan R (1981) Studies on desi and kabuli chickpea (Cicer arietinum L.) cultivars: Levels of protease inhibitors, levels of polyphenolic compounds and in vitro protein digestibility. J Food Sci 46: 1364–1367.
Snedecor GW, Cochran WC (1967) One way classification: Analysis of variance. In: Statistical methods, 6th ed. New Delhi: Oxford & IBH Publishing Co.
Duhan A, Chauhan BM, Punia D, Kapoor AC (1989) Phytic acid content of chickpea (Cicer arietinum) and black gram (Vigna mungo): Varietal differences and effect of domestic processing and cooking methods. J Sci Food Agric 49: 449–455.
Williams PC, Singh U (1987). The chickpea: Nutritional quality and the evaluation of quality in breeding programmes. In: Saxena MC, Singh KB (eds), The chickpea. Slough: CAB International.
Bassiri A, Nahapetian A (1977) Differences in concentrations and interrelationships of phytate, phosphorus, magnesium, calcium, zinc, and iron in wheat varieties grown under dry land and irrigated conditions. J Agric Food Chem 25: 1118–1122.
Miller GA, Youngs VL, Oplinger ES (1980) Environmental and cultivar effects on oat phytic acid concentration. Cereal Chem 57: 189–191.
Jood S, Chauhan BM, Kapoor AC (1989) Protein digestibility (in vitro) of chickpea and blackgram seeds as affected by domestic processing and cooking. Plant Foods Hum Nutr 39: 149–154.
Raboy V, Dickinson DB, Below FE (1984) Variation in seed total phosphorus, phytic acid, zinc, calcium, magnesium, and protein among lines ofGlycine max andG. soja. Crop Sci 24: 431–434.
Ritter MA, Morr CV, Thomas RL (1987) In vitro digestibility of phytate-reduced and phenolics-reduced soy protein isolates. J Food Sci 52: 325–327, 341.
Thompson LU, Serraino MR (1986) Effect of phytic acid reduction on rapeseed protein digestibility and amino acid absorption. J Agric Food Chem 34: 468–469.
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Chitra, U., Vimala, V., Singh, U. et al. Variability in phytic acid content and protein digestibility of grain legumes. Plant Food Hum Nutr 47, 163–172 (1995). https://doi.org/10.1007/BF01089266
- Grain legumes
- Phytic acid
- Protein digestibility
- Seed size