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Effects of heat treatment and germination on trypsin and chymotrypsin inhibitory activities in sorghum (Sorghum bicolor (L.) Moench) seeds

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Abstract

There was no appreciable change in proteinase inhibitory activity in sorghum upon dry heat treatment. However, moist heating reduced trypsin and chymotrypsin inhibitors to a greater degree. Germination (5 days) brought about complete reduction in proteinase inhibitory activity.

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References

  1. Salunkhe DK, Kadam SS, Chavan JK (1977) Nutritional quality of proteins in grain sorghum. Plant Foods Hum Nutr 27: 187–205

    Google Scholar 

  2. Rackis JJ, Wolf WJ, Baker CE (1986) Protease inhibitors in plant foods: Content and inactivation. Adv Exp Med Biol 199: 299–320

    PubMed  Google Scholar 

  3. Gallaher D, Schneeman BO (1984) Nutritional and metabolic response to plant inhibitors of digestive enzymes. Adv Exp Med Biol 177: 299–315

    PubMed  Google Scholar 

  4. Pattabirman TN (1986) Trypsin/Chymotrypsin inhibitors from millets. Adv Exp Med Biol 199: 439–450

    PubMed  Google Scholar 

  5. Salunkhe DK, Jadhav SJ, Kadam SS, Chavan JK (1983) Chemical, biochemical and biological significance of polyphenols in cereals and legumes. Crit Rev Food Sci Nutr 17: 277–305

    Google Scholar 

  6. Harishkumar PM, Virupaksha TK, Vithyathil PJ (1978) Sorghum proteinase inhibitors: Purification and some biochemical properties. Int J Pept Prot Res 12: 185–196

    Google Scholar 

  7. Harishkumar PM, Virupaksha TK, Vithyathil PJ (1979) Sorghum proteinase inhibitors 2. Mode of interaction with serine proteinases. Int J Pept Prot Res 13: 153–159

    Google Scholar 

  8. Sumathi S, Pattabiraman TN (1977) Natural plant enzyme inhibitors V. A trypsin/chymotrypsin inhibitor from Aocasia Macrorhiza tuber. Biochem Biophys Acta 485: 167–173

    PubMed  Google Scholar 

  9. Lowry OH, Roseberough NJ, Farr AL, Randall RJ (1951) Portein measurement with Folinphenol reagent. J Biol Chem 193: 265–275

    PubMed  Google Scholar 

  10. Al-Bakir AY, Sochde AG, Nacum IE (1981) Occurence and stability of trypsin inhibitors in Iraqi local legumes. J Agric Food Chem 30: 1184–1186

    Google Scholar 

  11. Hildrand DF, Hettiarachchy NN, Hymowitz T, Erdman JW Jr (1981) Electrophoretic separation and properties of winged bean seed trypsin inhibitors. J Sci Food Agric 32: 443–446

    Google Scholar 

  12. Chang CR, Tsen CC (1981) Characterization and heat stability of trypsin inhibitors from rye, triticale and wheat samples. Cereal Chem 58: 211–215

    Google Scholar 

  13. Kadam SS, Ghorphade PN, Adsule RN, Salunkhe DK (1986) Plant Foods Hum Nutr 36: 43–48

    Google Scholar 

  14. Saini HS (1989) Thermal stability of protease inhibitors in some cereals and legumes. Food Chem 32: 59–62

    Google Scholar 

  15. Ayyagari R, Narsinga BS, Roy DN (1989) Lectins, trypsin inhibitors and tannin in legumes and cereals and the effect of processing. Food Chem 34: 229–233

    Google Scholar 

  16. Babar VS, Chavan JK, Kadam SS (1988) Effects of heat treatments and germination on trypsin inhibitor activity and polyphenols in jack bean. Plant Foods Hum Nutr 38: 319–324

    PubMed  Google Scholar 

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Mulimani, V.H., Vadiraj, S. Effects of heat treatment and germination on trypsin and chymotrypsin inhibitory activities in sorghum (Sorghum bicolor (L.) Moench) seeds. Plant Food Hum Nutr 44, 221–226 (1993). https://doi.org/10.1007/BF01088316

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  • DOI: https://doi.org/10.1007/BF01088316

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